Morizane T, Satoh I, Kumagai N, Saito H, Iino A, Inagaki Y, Watanabe T, Tsuchimoto K, Tsuchiya M
J Immunol Methods. 1986 Jul 24;91(2):225-9. doi: 10.1016/0022-1759(86)90482-5.
A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8 and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.
一种适用于体外检测迟发型超敏反应的新型微玻璃管白细胞黏附抑制（LAI）试验已被开发用于人白细胞。通过细胞放射免疫测定法来确定单克隆试剂OKT4、OKT8和OKM1与经戊二醛或甲醛固定的玻璃黏附细胞的抗体结合，以此取代对黏附细胞的计数。在用OKT4试剂进行结核菌素纯蛋白衍生物（PPD）皮肤试验呈阳性的供体中可检测到对PPD的LAI反应性，但使用其他两种试剂时则检测不到。
