School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, P.O. Box 4059, Valparaíso, Chile.
School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, P.O. Box 4059, Valparaíso, Chile; Centro Regional de Estudios en Alimentos Saludables, Av. Universidad 330, Valparaíso, Chile.
Food Chem. 2019 Aug 1;288:102-107. doi: 10.1016/j.foodchem.2019.02.024. Epub 2019 Feb 14.
A new bi-enzymatic catalyst has been produced by precipitation and crosslinking (combi-CLEAs) of β-galactosidase and glucose isomerase for catalyzing the cascade reactions of lactose conversion into fructose, producing a lactose-fructose syrup (LFS). Glucose isomerase was chemically aminated to increase its reactive surface groups for favour the crosslinking step. The effect of β-galactosidase to glucose isomerase activity ratio and glutaraldehyde to protein mass ratio in combi-CLEAs production was evaluated. The selected combi-catalyst was successfully used in the production of fructose syrup from lactose in a single reaction vessel. The biocatalyst could be used at least in five sequential batches of LFS production, remaining fully stable after a total of 50 h of reaction, obtaining a product of constant quality. A robust bi-enzymatic catalyst was produced that can be repeatedly used in LFS production, an attractive mild sweetener for the dairy food industry.
一种新的双酶生物催化剂通过沉淀和交联（combi-CLEAs）β-半乳糖苷酶和葡萄糖异构酶来催化乳糖转化为果糖的级联反应，生产乳糖果糖糖浆（LFS）。葡萄糖异构酶经过化学氨化处理，增加了其反应性表面基团，有利于交联步骤。评估了 combi-CLEAs 生产中β-半乳糖苷酶与葡萄糖异构酶活性比和戊二醛与蛋白质质量比的影响。选择的复合催化剂成功地用于在单个反应容器中从乳糖生产果糖糖浆。该生物催化剂至少可以在五个连续批次的 LFS 生产中使用，在总共 50 小时的反应后仍保持完全稳定，获得了恒定质量的产物。生产出一种稳定的双酶生物催化剂，可重复用于 LFS 生产，是乳制品行业有吸引力的温和甜味剂。
