College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; College of Food Science and Technology, Jiangsu Food and Pharmaceutical Science College, Huaian 223005, Jiangsu, China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
Food Chem. 2019 Aug 1;288:368-376. doi: 10.1016/j.foodchem.2019.02.131. Epub 2019 Mar 11.
Exogenous Ca affects the phenolic metabolism and physiological indices of germinated wheat under ultraviolet-B (UV-B) radiation, but the mechanism is unclear. The current study applied exogenous Ca and Ca channel blocker LaCl to the germinated wheat under UV-B radiation to unravel the role of Ca. The results indicated that total phenolic content (TPC) of the 4-day germinated wheat under UV-B radiation with CaCl (UV-B+Ca) treatment significantly increased by 10.3% as compared to the UV-B treatment. Gene expression levels of p-coumarate 3-hydroxylase, cinnamic acid 4-hydroxylase and caffeic acid O-methyltransferase were positively correlated with the content of ferulic and p-coumaric acids, respectively. Exogenous Ca could significantly alleviate the membrane lipid peroxidation, activate the antioxidant enzymes and regulate the phytohormone level under UV-B radiation. This study suggested that exogenous Ca participated in the phenolic metabolism and physiological regulation in germinated wheat under UV-B radiation.
外源钙对紫外辐射下萌发小麦的酚类代谢和生理指标的影响，但机制尚不清楚。本研究在紫外辐射下对萌发小麦施加外源钙和钙通道阻滞剂 LaCl，以揭示钙的作用。结果表明，与单独紫外辐射处理相比，紫外+钙处理下的 4 天萌发小麦的总酚含量（TPC）显著增加了 10.3%。对 p-香豆酸 3-羟化酶、肉桂酸 4-羟化酶和咖啡酸 O-甲基转移酶的基因表达水平与阿魏酸和对香豆酸的含量呈正相关。外源钙可显著缓解紫外辐射下的膜脂过氧化，激活抗氧化酶并调节植物激素水平。本研究表明，外源钙参与了紫外辐射下萌发小麦的酚类代谢和生理调节。
