Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Mol Plant. 2019 Apr 1;12(4):597-602. doi: 10.1016/j.molp.2019.03.006. Epub 2019 Mar 19.
Crop breeding aims to generate pure inbred lines with multiple desired traits. Doubled haploid (DH) and genome editing using CRISPR/Cas9 are two powerful game-changing technologies in crop breeding. However, both of them still fall short for rapid generation of pure elite lines with integrated favorable traits. Here, we report the development of a Haploid-Inducer Mediated Genome Editing (IMGE) approach, which utilizes a maize haploid inducer line carrying a CRISPR/Cas9 cassette targeting for a desired agronomic trait to pollinate an elite maize inbred line and to generate genome-edited haploids in the elite maize background. Homozygous pure DH lines with the desired trait improvement could be generated within two generations, thus bypassing the lengthy procedure of repeated crossing and backcrossing used in conventional breeding for integrating a desirable trait into elite commercial backgrounds.
作物育种旨在生成具有多种所需性状的纯自交系。加倍单倍体（DH）和使用 CRISPR/Cas9 的基因组编辑是作物育种中两种强大的颠覆性技术。然而，它们都不能快速生成具有综合有利性状的纯精英系。在这里，我们报告了一种单倍体诱导介导的基因组编辑（IMGE）方法的开发，该方法利用携带针对所需农艺性状的 CRISPR/Cas9 盒的玉米单倍体诱导系授粉优良玉米自交系，并在优良玉米背景下生成基因组编辑的单倍体。具有所需性状改良的纯 DH 系可以在两代内产生，从而绕过传统育种中用于将理想性状整合到优良商业背景中的反复杂交和回交的漫长过程。
