Kondo E, Kanai K
Jpn J Med Sci Biol. 1986 Feb;39(1):9-20. doi: 10.7883/yoken1952.39.9.
The membrane fraction prepared from mouse peritoneal exudate cells was incubated with mycobacteria, staphylococci, or E. coli in acetate buffer of pH 5.6 to follow the fate of viable bacilli. The membrane fraction exhibited bactericidal effect on mycobacteria and staphylococci, but not on E. coli. The activity to kill mycobacteria, as well as the endogenous phospholipase A2 activity, of the membrane fraction was markedly enhanced by melittin, a basic peptide from bee venom, and inhibited by indomethacin and EDTA. The role of the enzyme activity in the bactericidal activity was discussed.
从小鼠腹腔渗出细胞制备的膜组分在pH 5.6的乙酸盐缓冲液中与分枝杆菌、葡萄球菌或大肠杆菌一起孵育，以追踪活菌的命运。该膜组分对分枝杆菌和葡萄球菌表现出杀菌作用，但对大肠杆菌没有作用。蜂毒中的一种碱性肽蜂毒素可显著增强该膜组分杀灭分枝杆菌的活性以及内源性磷脂酶A2的活性，而吲哚美辛和乙二胺四乙酸（EDTA）则可抑制这些活性。文中讨论了酶活性在杀菌活性中的作用。
