从生物源城市固体废物中单罐生物工艺生产乙醇。
Single pot bioprocessing for ethanol production from biogenic municipal solid waste.
机构信息
Bioengineering and Environmental Sciences Lab, CEEFF, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500007, Telangana, India.
Bioengineering and Environmental Sciences Lab, CEEFF, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500007, Telangana, India.
出版信息
Bioresour Technol. 2019 Jul;283:159-167. doi: 10.1016/j.biortech.2019.03.055. Epub 2019 Mar 11.
Burgeoning global energy demand and rapid diminution of fossil fuel reserves urged to seek for a sustainable energy source like bioethanol. Single pot bioprocessing (SPB) strategy employing in-house laccase, cellulase plus xylanase and amylase along with hexose and pentose sugar fermenting yeasts (Saccharomyces cerevisiae and Pichia stipitis) is designed in this study for ethanol production from biogenic municipal solid waste (BMSW). BMSW when subjected to simultaneous pretreatment and saccharification (SPS) resulted in 79.69% enzymatic digestibility and fared better compared to alkali pretreated counterparts (14.03%-51.10%). The maximum total sugar release in case of SPS was 146.9 g/L in 24 h. The maximum ethanol concentration of 5.24% (v/v) in 30 h was obtained from SPB of BMSW at 25% (w/v) solid loading. SPB for ethanol production from BMSW is an interesting and effective alternative to MSW going to landfill or incineration with an added perk of waste to wealth conversion.
蓬勃发展的全球能源需求和化石燃料储量的迅速减少，促使人们寻求像生物乙醇这样的可持续能源。本研究设计了一种采用内源性漆酶、纤维素酶加木聚糖酶和淀粉酶以及己糖和戊糖发酵酵母（酿酒酵母和毕赤酵母）的一锅法生物过程（SPB）策略，用于从生物城市固体废物（BMSW）中生产乙醇。BMSW 进行同步预处理和糖化（SPS）后，酶解率达到 79.69%，优于碱预处理的对应物（14.03%-51.10%）。在 SPS 的情况下，最大总糖释放量在 24 小时内达到 146.9 g/L。在 25%（w/v）固体负荷下，从 BMSW 的 SPB 中可在 30 小时内获得 5.24%（v/v）的最大乙醇浓度。与运往垃圾填埋场或焚烧的 MSW 相比，使用 SPB 从 BMSW 生产乙醇是一种有趣且有效的替代方法，除了废物转化为财富的额外好处外，还可以解决问题。
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