Olguin-Maciel Edgar, Larqué-Saavedra Alfonso, Lappe-Oliveras Patricia E, Barahona-Pérez Luis F, Alzate-Gaviria Liliana, Chablé-Villacis Rubí, Domínguez-Maldonado Jorge, Pacheco-Catalán Daniella, Ruíz Hector A, Tapia-Tussell Raúl
Renewable Energy Department, Yucatan Center for Scientific Research, Merida 97302, Mexico.
Natural Resource Department, Yucatan Center for Scientific Research, Merida 97205, Mexico.
Microorganisms. 2019 Oct 23;7(11):483. doi: 10.3390/microorganisms7110483.
Consolidated bioprocessing (CBP), which integrates biological pretreatment, enzyme production, saccharification, and fermentation, is a promising operational strategy for cost-effective ethanol production from biomass. In this study, the use of a native strain of (Bm-2) was evaluated for bioethanol production from in a CBP. The raw seed flour obtained from the ramon tree contained 61% of starch, indicating its potential as a raw material for bioethanol production. Quantitative assays revealed that the Bm-2 strain produced the amylase enzyme with activity of 193.85 U/mL. The Bm-2 strain showed high tolerance to ethanol stress and was capable of directly producing ethanol from raw flour at a concentration of 13 g/L, with a production yield of 123.4 mL/kg flour. This study demonstrates the potential of Bm-2 for starch-based ethanol production in a consolidated bioprocess to be implemented in the biofuel industry. The residual biomass after fermentation showed an average protein content of 22.5%, suggesting that it could also be considered as a valuable biorefinery co-product for animal feeding.
整合生物加工（CBP）将生物预处理、酶生产、糖化和发酵整合在一起，是一种从生物质中经济高效生产乙醇的有前景的操作策略。在本研究中，评估了本地菌株（Bm - 2）在整合生物加工中从[原料名称未给出]生产生物乙醇的情况。从拉蒙树获得的粗种子粉含有61%的淀粉，表明其作为生物乙醇生产原料的潜力。定量分析表明，Bm - 2菌株产生的淀粉酶活性为193.85 U/mL。Bm - 2菌株对乙醇胁迫具有高耐受性，能够直接从浓度为13 g/L的粗面粉中生产乙醇，产量为123.4 mL/kg面粉。本研究证明了Bm - 2在整合生物加工中用于基于淀粉的乙醇生产在生物燃料行业实施的潜力。发酵后的残余生物质平均蛋白质含量为22.5%，表明它也可被视为用于动物饲料的有价值的生物精炼副产品。
