Fu Xianguo, Yang Jing, Wu Xiaoyang, Lin Qifang, Zeng Yuli, Xia Qiaoqing, Cao Luoyuan, Huang Baoying, Huang Genbin
Laboratory of Cell and Molecular Biology, Ningde Municipal Hospital, Fujian Medical University, Ningde, Fujian, China.
Clin Invest Med. 2019 Mar 23;42(1):E21-E30. doi: 10.25011/cim.v42i1.32389.
The prevalence of migraines in the She population, a minority in China, is significantly higher than that in Han Chinese and other Asian populations. Two single nucleotide polymorphisms (SNPs) have been found to be associated with migraine susceptibility in the She population.
This study investigated four SNPs, identified in genome-wide association studies, within migraine-susceptible loci in Han Chinese for their association with migraine susceptibility in the She population.
Two-hundred unrelated migraine patients and 200 healthy controls were recruited. The SNPs examined included rs2651899 (PRDM16 ), rs2274316 (MEF2D ), rs7577262 (TRPM8) and rs11172113 (LRP1). Genotyping of the SNPs was performed by allele-specific polymerase chain reaction and direct sequencing.
No significant differences between the participants with migraines and controls (participants without migraines) were demonstrated in genotypes, alleles and allele carriage frequencies for the four SNPs. A subgroup analysis found that migraine with aura had a lower frequency of C allele positivity in rs2651899 than in healthy controls (59.6% vs. 74.5%, respectively; P < 0.034). Univariate analyses indicated that no genotype of the four SNPs had a significant association with migraines. Males had a lower risk of migraines, and advanced age was a significant risk factor for migraines in females.
The SNPs in four migraine susceptible loci in Han Chinese were not risk factors for migraines in a relatively small sample of the She population.
畲族是中国的一个少数民族，其偏头痛患病率显著高于汉族和其他亚洲人群。已发现两个单核苷酸多态性（SNP）与畲族偏头痛易感性相关。
本研究调查了在汉族全基因组关联研究中确定的四个位于偏头痛易感基因座内的SNP与畲族偏头痛易感性的关联。
招募了200名无亲缘关系的偏头痛患者和200名健康对照。检测的SNP包括rs2651899（PRDM16）、rs2274316（MEF2D）、rs7577262（TRPM8）和rs11172113（LRP1）。通过等位基因特异性聚合酶链反应和直接测序对SNP进行基因分型。
在四个SNP的基因型、等位基因和等位基因携带频率方面，偏头痛患者与对照组（无偏头痛参与者）之间未显示出显著差异。亚组分析发现，有先兆偏头痛患者rs2651899中C等位基因阳性频率低于健康对照（分别为59.6%和74.5%；P<0.034）。单因素分析表明，四个SNP的基因型与偏头痛均无显著关联。男性患偏头痛的风险较低，而高龄是女性偏头痛的一个显著风险因素。
在畲族相对较小的样本中，汉族四个偏头痛易感基因座中的SNP不是偏头痛的风险因素。
