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验证一种用于生乳和加工乳中碘定量的金标准方法,并研究其在不同乳品种类中的变化。

Validation of a gold standard method for iodine quantification in raw and processed milk, and its variation in different dairy species.

机构信息

Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Viale dell'Università 16, 35020 Legnaro (PD), Italy.

Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Viale dell'Università 16, 35020 Legnaro (PD), Italy.

出版信息

J Dairy Sci. 2019 Jun;102(6):4808-4815. doi: 10.3168/jds.2018-15946. Epub 2019 Mar 21.

DOI:10.3168/jds.2018-15946
PMID:30904296
Abstract

Adequate milk consumption significantly contributes to meeting the human iodine recommended daily intake, which ranges from 70 µg/d for infants to 200 µg/d for lactating women. The fulfilment of iodine recommended daily intake is fundamental to prevent serious clinical diseases such as cretinism in infants and goiter in adults. In the present study iodine content was measured in raw and processed commercial cow milk, as well as in raw buffalo, goat, sheep, and donkey milk. Iodine extraction was based on 0.6% (vol/vol) ammonia, whereas iodine detection and quantification were carried out through an inductively coupled plasma mass spectrometer analyzer. Among processed commercial cow milk, partially skimmed pasteurized milk had the greatest iodine content (359.42 µg/kg) and raw milk the lowest (166.92 µg/kg). With regard to the other dairy species, the greatest iodine content was found in raw goat milk (575.42 µg/kg), followed by raw buffalo (229.82 µg/kg), sheep (192.64 µg/kg), and donkey milk (7.06 µg/kg). Repeatability of milk iodine content, calculated as relative standard deviation of 5 measurements within a day or operator, ranged from 0.96 to 1.84% and 0.72 to 1.16%, respectively. The overall reproducibility of milk iodine content, calculated as relative standard deviation of 45 measurements across 3 d of analyses and 3 operators, was 4.01%. These results underline the precision of the proposed analytical method for the determination of iodine content in milk.

摘要

摄入足够的牛奶可显著有助于满足人体碘的推荐日摄入量,该摄入量范围为婴儿 70μg/d,哺乳期妇女 200μg/d。满足碘的推荐日摄入量对于预防严重的临床疾病(如婴儿克汀病和成人甲状腺肿)至关重要。在本研究中,我们测量了生牛奶和加工商业牛奶(全脂、部分脱脂和巴氏杀菌),以及生水牛、山羊、绵羊和驴奶中的碘含量。碘的提取基于 0.6%(体积/体积)的氨,而碘的检测和定量则通过电感耦合等离子体质谱分析仪进行。在加工商业牛奶中,部分脱脂巴氏杀菌牛奶的碘含量最高(359.42μg/kg),生牛奶的碘含量最低(166.92μg/kg)。至于其他乳制品,生羊奶的碘含量最高(575.42μg/kg),其次为生水牛(229.82μg/kg)、绵羊(192.64μg/kg)和驴奶(7.06μg/kg)。牛奶碘含量的重复性,即每天 5 次测量或操作人员内的相对标准偏差,范围分别为 0.96%至 1.84%和 0.72%至 1.16%。牛奶碘含量的整体重现性,即 3 天分析和 3 名操作人员的 45 次测量的相对标准偏差,为 4.01%。这些结果突出了所提出的牛奶碘含量测定分析方法的精密度。

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