Department of Stomatognathic Function and Occlusal Reconstruction, Graduate School of Biomedical Sciences, Tokushima University, Japan.
Department of Oral Molecular Pathology, Graduate School of Biomedical Sciences, Tokushima University, Japan.
Neurosci Lett. 2019 Jun 11;703:132-138. doi: 10.1016/j.neulet.2019.03.031. Epub 2019 Mar 20.
Many trigeminal neuropathic pain patients suffer severe chronic pain. The neuropathic pain might be related with cross-excitation of the neighboring neurons and satellite glial cells (SGCs) in the sensory ganglia and increasing the pain signals from the peripheral tissue to the central nervous system. We induced trigeminal neuropathic pain by infraorbital nerve constriction injury (IONC) in Sprague-Dawley rats. We tested cytokine (CXCL2 and IL-10) levels in trigeminal ganglia (TGs) after trigeminal neuropathic pain induction, and the effect of direct injection of the anti-CXCL2 and recombinant IL-10 into TG. We found that IONC induced pain behavior. Additionally, IONC induced satellite glial cell activation in TG and cytokine levels of TGs were changed after IONC. CXCL2 levels increased on day 1 of neuropathic pain induction and decreased gradually, with IL-10 levels showing the opposite trend. Recombinant IL-10 or anti-CXCL2 injection into TG decreased pain behavior. Our results show that IL-10 or anti-CXCL2 are therapy options for neuropathic pain.
许多三叉神经病理性疼痛患者患有严重的慢性疼痛。这种神经病理性疼痛可能与感觉神经节中相邻神经元和卫星神经胶质细胞（SGC）的交叉兴奋以及外周组织向中枢神经系统传递疼痛信号的增加有关。我们通过眶下神经缩窄损伤（IONC）诱导 Sprague-Dawley 大鼠三叉神经病理性疼痛。我们检测了三叉神经节（TGs）中细胞因子（CXCL2 和 IL-10）水平，在诱导三叉神经病理性疼痛后，以及直接将抗-CXCL2 和重组 IL-10 注射到 TG 中的效果。我们发现 IONC 诱导了疼痛行为。此外，IONC 诱导了 TG 中卫星神经胶质细胞的激活，并且 IONC 后 TG 中的细胞因子水平发生了变化。CXCL2 水平在神经病理性疼痛诱导的第 1 天增加，并逐渐降低，而 IL-10 水平则呈现相反的趋势。将重组 IL-10 或抗-CXCL2 注射到 TG 中可以减轻疼痛行为。我们的结果表明，IL-10 或抗-CXCL2 是治疗神经病理性疼痛的选择。
