Department of Physiological Sciences Santa Casa de Sao Paulo School of Medical Sciences, School of Medicine, São Paulo, SP, Brazil.
J Ethnopharmacol. 2019 Jun 12;237:74-80. doi: 10.1016/j.jep.2019.03.050. Epub 2019 Mar 21.
Pimpinella anisum is used in traditional medicine because of its pharmacological properties which include cardiovascular action. However, no scientific information supports this use.
This study investigated the effects of Pimpinella on arterial blood pressure (BP) and its pharmacological mechanism of action.
Pimpinella seeds were extracted with water, concentrated and freeze-dried yielding the aqueous extract (AE). A non-invasive BP assessment method was used (via the caudal artery) on Wistar, Wistar Kyoto, SHRs and rats that were submitted to high intake of dietary salt. Direct BP and heart rate were evaluated in Wistar rats in the absence or presence of atropine, L-NAME and angiotensin II. Spontaneous diuresis and the effect of AE on depolarized portal vein of Wistar rats was also examined.
The data revealed that AE reduced BP in all groups evaluated and its effects were not due to diuretic, sympatholytic or parasympathomimetic actions. Additionally, it was shown that AE does not act as an angiotensin receptor blocker and does not induce hypotension by reducing vascular resistance induced by oxide nitric. In the depolarized portal vein, AE inhibited calcium influx, which indicates that AE acts as calcium channel blocker.
This study validates the cardiovascular actions of Pimpinella and characterizes the hypotensive effects of Pimpinella that are related to the blockade of calcium channels.
由于具有心血管作用等药理学特性，水芹被用于传统医学。然而，没有科学信息支持这种用途。
本研究旨在调查水芹对动脉血压（BP）的影响及其作用机制。
用水提取、浓缩并冻干水芹种子，得到水提取物（AE）。采用非侵入性的尾部动脉血压评估方法（通过尾部动脉），对 Wistar、Wistar Kyoto、SHR 和高盐饮食的大鼠进行评估。在 Wistar 大鼠中评估 AE 在不存在或存在阿托品、L-NAME 和血管紧张素 II 时对直接 BP 和心率的影响。还检查了 AE 对 Wistar 大鼠去极化门静脉的自发利尿作用和影响。
数据显示，AE 降低了所有评估组的 BP，其作用不是由于利尿、交感神经抑制或副交感神经拟似作用。此外，还表明 AE 不作为血管紧张素受体阻滞剂，并且不会通过降低由氧化氮诱导的血管阻力来引起低血压。在去极化门静脉中，AE 抑制钙内流，这表明 AE 作为钙通道阻滞剂起作用。
本研究验证了水芹的心血管作用，并描述了与钙通道阻断相关的水芹的降压作用。
