Es-Safi Imane, Mechchate Hamza, Amaghnouje Amal, Elbouzidi Amine, Bouhrim Mohamed, Bencheikh Noureddine, Hano Christophe, Bousta Dalila
Laboratory of Biotechnology, Environment, Agrifood, and Health, Faculty of Sciences Dhar el Mahraz, University of Sidi Mohamed Ben Abdellah, Fez 30050, Morocco.
Laboratory of Bioresources, Biotechnology, Ethnopharmacology, and Health, Faculty of Sciences, Mohammed First University, Oujda 60040, Morocco.
Plants (Basel). 2021 Jul 30;10(8):1573. doi: 10.3390/plants10081573.
Mental disorders are psychological symptoms that impact multiple areas of an individual's life. Depression and anxiety are chronic illnesses described as the most prevalent stress-related mood disorders that cause injury and early death. In Morocco, Anise " L." is one of the most traditionally used condiment plants, which has long been used to cure various illnesses and in phytotherapy. The present study was designed to investigate the antidepressant, anxiolytic, and memory impact of the total extract of (PATE) at the doses of 100 and 200 mg/kg, using the Forced Swimming Test (FST), Tail Suspension Test (TST), Open Field Test (OFT), and Light-Dark Box Test (LDBT) as an experimental paradigm of anxiety and depression, and Novel Object Recognition Test (NORT) and the Morris Water Maze Test (MWMT) as memory tests on Swiss albino mice. The tests were carried out on the 1st, 7th, 14th, and the 21st days of the study, and the extract groups were compared with normal controls and positive controls (receiving bromazepam and paroxetine at the doses of 1 mg/kg and 11.5 mg/kg for anxiety and depression, respectively). The daily oral gavage of the mice by the PATE induced a significant anxiolytic and antidepressant-like effect by shortening immobility time and decreasing downtime in the different tests. PATE at both doses was shown to have no impact on memory following the NORT and MWM tests. Different compounds, such as gallic acid, catechin, chlorogenic acid, caffeic acid, oleuropein, -coumaric acid, trans-4-hydroxy-3-methoxycinnamic acid, myricetin, and quercetin, were identified during the phytochemical analysis carried out using HPLC analysis. This research supports and promotes the extract's traditional use, suggesting its use as a phytomedicine against depression and anxiety, and calls for further research to clarify its mode of action.
精神障碍是影响个体生活多个方面的心理症状。抑郁症和焦虑症是慢性疾病，被描述为最常见的与压力相关的情绪障碍，会导致伤害和过早死亡。在摩洛哥，茴芹“L.”是最传统使用的调味品植物之一，长期以来一直用于治疗各种疾病和植物疗法。本研究旨在使用强迫游泳试验（FST）、悬尾试验（TST）、旷场试验（OFT）和明暗箱试验（LDBT）作为焦虑和抑郁的实验范式，以及新物体识别试验（NORT）和莫里斯水迷宫试验（MWMT）作为对瑞士白化小鼠的记忆测试，来研究茴芹总提取物（PATE）在100和200mg/kg剂量下的抗抑郁、抗焦虑和对记忆的影响。试验在研究的第1、7、14和21天进行，提取物组与正常对照组和阳性对照组（分别以1mg/kg和11.5mg/kg的剂量接受溴西泮和帕罗西汀治疗焦虑和抑郁）进行比较。通过缩短不同试验中的不动时间和减少静止时间，PATE每日口服灌胃对小鼠产生了显著的抗焦虑和抗抑郁样作用。在NORT和MWM试验后，两种剂量的PATE均显示对记忆无影响。在使用HPLC分析进行的植物化学分析过程中，鉴定出了不同的化合物，如没食子酸、儿茶素、绿原酸、咖啡酸、橄榄苦苷、对香豆酸、反式-4-羟基-3-甲氧基肉桂酸、杨梅素和槲皮素。本研究支持并推广了该提取物的传统用途，表明其可作为抗抑郁和抗焦虑的植物药，并呼吁进一步研究以阐明其作用方式。
