College of Agronomy, Northwest A&F University, Yangling, 712100, Shanxi, China.
College of Horticulture, Northwest A&F University, Yangling, 712100, Shanxi, China.
Gene. 2019 Jun 5;700:149-162. doi: 10.1016/j.gene.2019.02.103. Epub 2019 Mar 21.
Class III peroxidases are classical secretory plant peroxidases belonging to a large multi-gene family. Class III peroxidases are involved in various physical processes and the response to biotic and abiotic stress to protect plants from environmental adversities. In this study, 151 BdPrx genes were identified using HMM and Blastp program. According to their physical location, the 151 BdPrx genes were mapped on five chromosomes. The results of Gene Structure Display Serve and MEME revealed that BdPrxs in the same subgroup shared similar gene structure, and their protein sequences were highly conserved. Based on the analysis of evolutionary relationships and Ka/Ks, 151 BdPrx genes were divided into 15 subgroups, they have undergone purifying selection. In addition, the result of GO annotation showed that 100% of the BdPrxs participated in antioxidant. The protein-protein interaction network was constructed using the orthology-based method, found that 66 BdPrxs were involved in the regulatory network and 183 network branches were identified. Furthermore, analysis of the transcriptome data indicated that the BdPrx genes responded to low concentration of exogenous phytohormones and exhibited different levels of expression in the different tissues. Subsequently, 19 genes were selected for quantitative real-time PCR and found to be mainly expressed in the roots, might preferentially respond to hydrogen peroxide and gibberellin. Our results provide a foundation for further evolutionary and functional study of Prx genes in B. distachyon.
III 类过氧化物酶是经典的分泌型植物过氧化物酶，属于一个大型多基因家族。III 类过氧化物酶参与各种物理过程和对生物及非生物胁迫的反应，以保护植物免受环境逆境的影响。在这项研究中，使用 HMM 和 Blastp 程序鉴定了 151 个 BdPrx 基因。根据它们的物理位置，将 151 个 BdPrx 基因映射到五个染色体上。基因结构显示服务和 MEME 的结果表明，同一亚组中的 BdPrxs 具有相似的基因结构，它们的蛋白质序列高度保守。基于进化关系和 Ka/Ks 的分析，将 151 个 BdPrx 基因分为 15 个亚组，它们经历了纯化选择。此外，GO 注释的结果表明，100%的 BdPrxs 参与了抗氧化。使用基于同源性的方法构建蛋白质-蛋白质相互作用网络，发现 66 个 BdPrxs 参与了调控网络，鉴定出 183 个网络分支。此外，对转录组数据的分析表明，BdPrx 基因对外源植物激素的低浓度有反应，并在不同组织中表现出不同水平的表达。随后，选择了 19 个基因进行定量实时 PCR 分析，发现它们主要在根部表达，可能优先响应过氧化氢和赤霉素。我们的研究结果为进一步研究 B. distachyon 中 Prx 基因的进化和功能提供了基础。
