Oil Crops Research Institute , Chinese Academy of Agricultural Sciences , Wuhan 430062 , PR China.
Key Laboratory of Biology and Genetic Improvement of Oil Crops , Ministry of Agriculture , Wuhan 430062 , PR China.
J Agric Food Chem. 2019 Apr 24;67(16):4595-4602. doi: 10.1021/acs.jafc.8b06810. Epub 2019 Apr 3.
Aflatoxin B (AFB), is a type I carcinogen that is one of the strongest naturally occurring aflatoxins and can be injurious to humans and livestock upon ingestion, inhalation, or skin contact, with carcinogenic and mutagenic effects. It causes significant hazardous effects to the food- and animal-production industries. We found a bacterial strain, 3J2MO, that degraded AFB well, and here we tested and characterized its AFB-degradation ability. The strain degraded about 93.82% of the AFB after incubation for 48 h in Luria-Bertani (LB) medium at 37 °C with a final concentration of 100 ppb and an inoculation quantity of 1 × 10 cfu/mL. High-performance liquid chromatography-fluorescence detection (HPLC-FLD) was used to determine AFB amounts. The maximum degradation rates were 89.23% at pH 8.5; 55.78% at an inoculation quantity of 1 × 10 cfu/mL; and 71.50 and 71.21% at 34 and 37 °C, respectively. Treatment with sucrose and soluble starch as carbon sources and beef extract and ammonium acetate as nitrogen sources stimulated the degradation rate. Mg and Ca ions were activators for AFB degradation; however, Mn, Fe, Zn, and Cu were strong inhibitors. This bacterial strain has potential in bioremediation and the detoxification of aflatoxin contamination for biocontrol strategies in both agricultural products and food-industry matrices.
黄曲霉毒素 B（AFB）是一种 I 类致癌物，是最强的天然存在的黄曲霉毒素之一，摄入、吸入或皮肤接触都会对人类和牲畜造成伤害，具有致癌和致突变作用。它对食品和动物生产行业造成了重大的危险影响。我们发现了一种能很好地降解 AFB 的细菌菌株 3J2MO，在这里我们测试并表征了其 AFB 降解能力。该菌株在 37°C 下用终浓度为 100ppb 和接种量为 1×10 cfu/mL 的 LB 培养基中孵育 48 小时后，可降解约 93.82%的 AFB。高效液相色谱-荧光检测（HPLC-FLD）用于测定 AFB 含量。最大降解率分别为 pH8.5 时 89.23%；接种量为 1×10 cfu/mL 时 55.78%；34°C 和 37°C 时分别为 71.50%和 71.21%。用蔗糖和可溶性淀粉作为碳源，用牛肉膏和乙酸铵作为氮源处理可提高降解率。Mg 和 Ca 离子是 AFB 降解的激活剂；然而，Mn、Fe、Zn 和 Cu 是强烈的抑制剂。该菌株在农产品和食品工业基质的生物防治策略中具有生物修复和脱毒黄曲霉毒素污染的潜力。
