Baran V, Petrík P, Federic F, Skarda R, Lenhardt L
Vet Med (Praha). 1986 Jul;31(7):393-402.
A sterile polyethylene device was introduced in the milk cistern of two mammary quarters of forty dairy cows. The cows were divided into three groups according to the length of exposure of the device in the cistern (3 days, 14 days, 365 days). The somatic cell counts were studied for a year in the first 10 ml fraction of milk and an increase in the somatic cell counts was found in the quarters having the intramammary device, as compared with the control quarters (having no devices). This difference was marked during lactation and prior to the onset of drying off. For the reduction in the frequency of occurrence of new natural intramammary infections, the activity of the device against S. aureus was limited and no activity against S. agalactiae was proved. The proportions of polymorphonuclears, round-cell elements and macrophages were histologically studied and compared for the udder quarters with and without the intramammary body in correlation with the time of exposure of the device to the milk cistern milieu. The most marked differences in favour of the udder quarters with the intramammary device were recorded in the alveoli containing more cells with a significant proportion of polymorphonuclear leucocytes. Small differences were found in the interstitial and subepithelial zone of the milk cistern. The activity of acid and alkaline phosphatase and adenosine triphosphatase was histochemically determined in the tissue structures of the udder and was found not to change under the influence of the device. Leucocytes and macrophages adhering to the surface of the body were observed under scanning electron microscope.
将一个无菌聚乙烯装置引入40头奶牛两个乳腺乳池。根据装置在乳池中的放置时间（3天、14天、365天）将奶牛分为三组。对最初10毫升乳汁中的体细胞计数进行了为期一年的研究，发现装有乳房内装置的乳腺乳池与对照乳池（未装装置）相比，体细胞计数有所增加。这种差异在泌乳期和干奶期开始前很明显。对于减少新的自然乳房内感染的发生频率，该装置对金黄色葡萄球菌的活性有限，且未证明对无乳链球菌有活性。对有和没有乳房内装置的乳腺乳池进行了组织学研究，比较了多形核细胞、圆形细胞成分和巨噬细胞的比例，并与装置暴露于乳池环境的时间相关联。在含有较多细胞且多形核白细胞比例显著的肺泡中，记录到装有乳房内装置的乳腺乳池有最明显的差异。在乳池的间质和上皮下区域发现了微小差异。通过组织化学方法测定了乳房组织结构中酸性磷酸酶、碱性磷酸酶和三磷酸腺苷酶的活性，发现其在装置的影响下没有变化。在扫描电子显微镜下观察了附着在装置表面的白细胞和巨噬细胞。
