Machatková M, Jurmanová K, Snejdar V
Vet Med (Praha). 1986 Jul;31(7):415-22.
The continual cell lines of bovine kidneys MDBK and AUBEK, and porcine kidneys RPD and IBRS, spontaneously infected with Mycoplasma arginini and Acholeplasma laidlawii, were decontaminated by the method of selective elimination. Two elimination procedures were modified to be used for the decontamination: one based on the reduction of infection by the light treatment of the cultures, the other based on the selection of mycoplasma-free cell population through cell clonation. On the basis of a long-continued control of the cell clones a methodical procedure of the preparation of mycoplasma-free cell lines was worked out.
持续感染精氨酸支原体和莱氏无胆甾原体的牛肾细胞系MDBK和AUBEK，以及猪肾细胞系RPD和IBRS，通过选择性消除法进行净化处理。改进了两种消除程序用于净化处理：一种是基于通过对培养物进行光照处理来减少感染，另一种是基于通过细胞克隆选择无支原体的细胞群体。在对细胞克隆进行长期监测的基础上，制定了一套制备无支原体细胞系的系统程序。
