Kirkeby S, Moe D
Acta Histochem. 1986;79(1):115-21. doi: 10.1016/S0065-1281(86)80108-8.
The effects of aldehyde fixation on tissue proteins was studied using SDS-polyacrylamide gel electrophoresis. Fixation with low concentrations of formaldehyde or glutaraldehyde had only a slight effect on the protein banding pattern. On the other hand, most of the original protein bands were absent after a short treatment with mixtures of formaldehyde and glutaraldehyde. Spectral absorption measurements showed a stronger absorption at both gamma = 280 nm and gamma = 235 nm with the glutaraldehyde-formaldehyde mixture than with glutaraldehyde alone.
使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳研究了醛固定对组织蛋白质的影响。用低浓度甲醛或戊二醛固定对蛋白质条带模式只有轻微影响。另一方面，用甲醛和戊二醛混合物短时间处理后，大部分原始蛋白质条带消失。光谱吸收测量表明，与单独使用戊二醛相比，戊二醛-甲醛混合物在γ = 280 nm和γ = 235 nm处的吸收更强。
