Simoni I C, Mello M L
Acta Histochem. 1986;79(1):97-105. doi: 10.1016/S0065-1281(86)80106-4.
Hycanthone is a drug used for the clinical treatment of schistosomiasis, although inducing chromosomes breaks and mutagenesis when it enters the DNA double helix. Based on visual observation of characteristics of basophilia and anisotropy after toluidine blue staining, a preferential binding of hycanthone to heterochromatin could be demonstrated for the nuclei of the Malpighian tubules of Triatoma infestans. In other cellular systems like cattle kidney cells in culture, plant cells, and mouse lymphocytes, the drug could be demonstrated to bind heterochromatin and euchromatin, irrespective of the packing state of the latter. When penetrating the various heterochromatin types (with the exception of T. infestans), the drug induced a chromatin loosening that could favor incidence of chromatin breaks. The variation of hycanthone binding to DNA in different cell types is possibly related to differences in composition, stereo-arrangement and stability of the DNA-protein complexes involved.
海恩酮是一种用于临床治疗血吸虫病的药物，尽管它在进入DNA双螺旋时会诱导染色体断裂和诱变。基于对甲苯胺蓝染色后嗜碱性和各向异性特征的视觉观察，可证明海恩酮优先结合于侵扰锥蝽马氏管细胞核的异染色质。在其他细胞系统中，如培养的牛肾细胞、植物细胞和小鼠淋巴细胞中，该药物可结合异染色质和常染色质，而与后者的包装状态无关。当穿透各种异染色质类型时（侵扰锥蝽除外），该药物会诱导染色质松弛，这可能有利于染色质断裂的发生。海恩酮在不同细胞类型中与DNA结合的差异可能与所涉及的DNA - 蛋白质复合物的组成、立体排列和稳定性差异有关。
