Babaei Faezeh, Ebrahimi Shahmabadi Hasan, Rajabi Mohammad Reza, Haddad Kashani Hamed, Izadpanah Fatemeh
Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran.
Department of Microbiology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Asian Pac J Cancer Prev. 2019 Mar 26;20(3):723-726. doi: 10.31557/APJCP.2019.20.3.723.
Background: Cisplatin (Cispt) is a common anticancer drug for the treatment of several malignancies, including hepatocarcinoma. However, this drug suffers from instability in aqueous solutions. The study aimed to evaluate cisplatin efficacy on HepG2 and E. coli cells under an acidic condition. Methods: Acidic Cispt was prepared via incubation in acidic condition (pH=2) for a month duration. The chemical structure of the acidic Cispt was evaluated by using Fourier Transform Infrared Spectroscopy (FTIR) method. The cytotoxicity of the standard and acidic Cispt were then determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and minimum inhibitory concentration (MIC) assays on HepG2 and E. coli cells, respectively. Results: After preparing of acidic Cispt, its chemical structure was determined by FTIR method. In addition, cytotoxicity effects of Cispt in the standard and acidic forms were calculated 58 ± 2.9 and 65 ± 3.25 μM, respectively. MIC results also confirmed the results of MTT assay. MIC results for the standard and acidic Cispt were estimated 9.5 ± 0.47 and 9.8 ± 0.49 μM, respectively. Conclusion: Preparing Cispt in acidic condition not only did not degrade the drug, but also kept the potency of the drug approximately equal to parent drug. Regarding the instability issues of Cispt, keeping Cispt in acidic condition could be a promising approach to preserve its efficacy.
顺铂（Cispt）是一种用于治疗多种恶性肿瘤（包括肝癌）的常用抗癌药物。然而，这种药物在水溶液中不稳定。本研究旨在评估酸性条件下顺铂对HepG2细胞和大肠杆菌细胞的疗效。方法：通过在酸性条件（pH = 2）下孵育一个月制备酸性顺铂。使用傅里叶变换红外光谱（FTIR）法评估酸性顺铂的化学结构。然后分别通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）和最低抑菌浓度（MIC）试验测定标准和顺铂对HepG2细胞和大肠杆菌细胞的细胞毒性。结果：制备酸性顺铂后，通过FTIR法确定其化学结构。此外，计算出标准和顺铂形式的顺铂细胞毒性效应分别为58±2.9和65±3.25μM。MIC结果也证实了MTT试验的结果。标准和顺铂的MIC结果分别估计为9.5±0.47和9.8±0.49μM。结论：在酸性条件下制备顺铂不仅不会使药物降解，而且能使药物效力与原药大致相当。鉴于顺铂的不稳定性问题，将顺铂置于酸性条件下可能是一种保持其疗效的有前景的方法。
