Scarfone E, Favre D, De Camilli P, Sans A
C R Acad Sci III. 1986;302(15):567-72.
The presence and localization of synapsin I, a neuron-specific phosphoprotein, was investigated in the cat vestibular epithelium, using a rabbit antisynapsin I anti-serum. The staining was performed by immunofluorescence or by a peroxidase-antiperoxidase (PAP) technique. A strong immunoreactivity was observed with both methods. This immunoreactivity appeared as spherical patches distributed in the lower part of the epithelium. This distribution pattern is very similar to that of the efferent synaptic endings which form axodendritic synapses with the afferent nerve chalice of type I hair cells, or axosomatic synapses with type II hair cells. Some of the nerve chalices were also labelled; in this case, the immunoreactivity was more evident with PAP staining. These results thus suggest the presence of large amounts of synapsin I in the vestibular efferent nerve endings. These endings are known to be filled with numerous synaptic vesicles. This localization of synapsin I is well correlated with previous work that report a close association between synapsin I and small synaptic vesicles. The presence of synapsin I in sensory endings such as the afferent nerve chalices was unexpected and is under investigation.
利用兔抗突触素I抗血清，研究了神经元特异性磷蛋白突触素I在猫前庭上皮中的存在情况及其定位。通过免疫荧光或过氧化物酶-抗过氧化物酶（PAP）技术进行染色。两种方法均观察到强烈的免疫反应性。这种免疫反应性表现为分布在上皮下部的球形斑块。这种分布模式与传出突触末梢的分布模式非常相似，传出突触末梢与I型毛细胞的传入神经杯形成轴-树突触，或与II型毛细胞形成轴-体突触。一些神经杯也被标记；在这种情况下，PAP染色的免疫反应性更明显。因此，这些结果表明前庭传出神经末梢中存在大量突触素I。已知这些末梢充满了大量突触小泡。突触素I的这种定位与先前报道突触素I与小突触小泡密切相关的研究结果高度相关。在诸如传入神经杯等感觉末梢中发现突触素I是出乎意料的，目前正在对此进行研究。
