Key Laboratory for Advanced Materials, School of Chemistry and Molecular Engineering East, East China University of Science and Technology, Shanghai, P. R. China.
School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, P. R. China.
Electrophoresis. 2019 Aug;40(16-17):2112-2116. doi: 10.1002/elps.201900096. Epub 2019 May 2.
Nanopore is a single-molecule analysis method which also employed electrophoresis has achieved promising single-molecule detections. In this study, we designed two kinds of confined spaces by fabricating solid-state nanopores with desirable diameters to study the structured single-strand DNA of C-rich quadruplex. For the nanopore whose diameter is larger than the quadruplex size, the DNA molecule could directly translocate through the nanopore with extremely high speed. For the nanopore whose diameter is smaller than the quadruplex size, DNA molecule which is captured by nanopore could return to the solution without translocation or unzip the quadruplex structure into single-strand and then pass the nanopore. This study certifies that choosing a suitable sensing interface is the vital importance of observing detailed single-molecule information. The solid-state nanopores hold the great potential to study the structural dynamics of quadruplex DNA molecule.
纳米孔是一种单分子分析方法，同样采用电泳技术，已经实现了有前途的单分子检测。在这项研究中，我们通过制造具有所需直径的固态纳米孔设计了两种限制空间，以研究富含 C 的四链体的结构化单链 DNA。对于直径大于四链体大小的纳米孔，DNA 分子可以以极高的速度直接穿过纳米孔。对于直径小于四链体大小的纳米孔，被纳米孔捕获的 DNA 分子可以在不迁移或不解开四链体结构成单链的情况下返回溶液，然后通过纳米孔。这项研究证明选择合适的传感界面对于观察详细的单分子信息至关重要。固态纳米孔具有研究四链体 DNA 分子结构动力学的巨大潜力。
