将新鲜粪便与冻干和冷冻粪便培养物作为接种物用于预防雏鸡沙门氏菌感染的比较。

Comparison of Fresh Feces with Lyophilized and Frozen Cultures of Feces as Inocula to Prevent Salmonella Infection in Chicks.

作者信息

Pivnick H, Blanchfield B, Rigby C, Ormsby E

机构信息

Bureau of Microbial Hazards, Food Directorate, Health Protection Branch, Department of National Health and Welfare, Ottawa, Ontario, Canada, K1A 0L2 and Agriculture Canada, Animal Disease Research Institute, Nepean P.O. Box 11300, Station H, Nepean, Ontario, Canada, K2H 8P9 and Division of Food Statistics and Operational Planning, Food Directorate, Health Protection Branch, Department of National Health and Welfare, Ottawa, Ontario, Canada, K1A 0L2.

出版信息

J Food Prot. 1982 Nov;45(13):1188-1194. doi: 10.4315/0362-028X-45.13.1188.

DOI:10.4315/0362-028X-45.13.1188

PMID:30913641

Abstract

Mixed treatment cultures obtained by inoculating anaerobic medium with chicken feces were administered to 0 to 1 d-old chicks in their drinking water. Three types of treatment cultures (all fourth passage) were compared: FFC (fresh fecal cultures started with inocula of fresh feces followed by four daily, uninterrupted serial subcultures), LFC (cultures started with inocula obtained by lyophilizing third-passage cultures), and FrFC (cultures started with inocula obtained by freezing third-passage cultures). Protection of treated chicks against infection by Salmonella typhimurium was assessed by challenging the chicks via their drinking water 2 d after treatment and culturing their ceca 8 to 9 d later, or at weekly intervals for 7 weeks. FFC protected chicks against infection more consistently than LFC or FrFC. However, some LFC and FrFC were as protective as FFC (α= .05). Treatment with cultures did not increase mortality or decrease weight gain.

摘要

将通过用鸡粪接种厌氧培养基获得的混合处理培养物添加到0至1日龄雏鸡的饮用水中。比较了三种类型的处理培养物（均为第四代传代培养物）：FFC（以新鲜粪便接种物开始的新鲜粪便培养物，随后进行连续四天的不间断连续传代培养）、LFC（以冻干第三代传代培养物获得的接种物开始的培养物）和FrFC（以冷冻第三代传代培养物获得的接种物开始的培养物）。在处理后2天通过雏鸡的饮用水对其进行攻击，并在8至9天后或每周一次共7周培养它们的盲肠，以此评估经处理的雏鸡对鼠伤寒沙门氏菌感染的抵抗力。FFC比LFC或FrFC更能持续保护雏鸡免受感染。然而，一些LFC和FrFC的保护效果与FFC相同（α = 0.05）。用培养物进行处理不会增加死亡率或降低体重增加。