Gores P F, Sutherland D E, Platt J L, Bach F H
J Immunol. 1986 Sep 1;137(5):1482-5.
Culture of pancreatic islets reduces their immunogenicity and results in prolonged graft survival after allotransplantation. The mechanism by which immunogenicity is reduced by culture is not known, but it has been suggested that prolonged graft survival is the result of the depletion of Ia+ cells from the graft. We studied the effect of eliminating Ia+ cells from islets before allotransplantation. Freshly isolated islets were incubated with anti-Ia serum plus complement or with monoclonal antibody to IAk plus complement or were left untreated before transplantation beneath the renal capsule of diabetic recipients. Incubation with anti-Ia serum plus complement eliminated intra-islet IA+ cells as demonstrated by indirect immunofluorescence staining. Incubation with monoclonal antibody to IAk plus complement significantly reduced but did not eliminate IA+ cells. Neither pretreatment regimen influenced survival of islet allografts placed beneath the renal capsule. However, untreated islets injected into the portal circulation were rejected in a low percentage of cases. We conclude that decreased immunogenicity observed after culture is not due solely to the depletion of Ia+ cells and that the site of engraftment has an important impact on graft survival.
胰岛培养可降低其免疫原性，并使同种异体移植后的移植物存活时间延长。培养降低免疫原性的机制尚不清楚，但有人提出移植物存活时间延长是移植物中Ia⁺细胞耗竭的结果。我们研究了在同种异体移植前从胰岛中清除Ia⁺细胞的效果。将新鲜分离的胰岛与抗Ia血清加补体或与抗IAk单克隆抗体加补体一起孵育，或在移植到糖尿病受体的肾被膜下之前不进行处理。如间接免疫荧光染色所示，与抗Ia血清加补体孵育可消除胰岛内的IA⁺细胞。与抗IAk单克隆抗体加补体孵育可显著减少但不能消除IA⁺细胞。两种预处理方案均未影响置于肾被膜下的胰岛同种异体移植物的存活。然而，注入门静脉循环的未处理胰岛在少数情况下被排斥。我们得出结论，培养后观察到的免疫原性降低并非仅由于Ia⁺细胞的耗竭，且植入部位对移植物存活有重要影响。
