Bhattarai Sheeva, Framstad Tore, Nielsen Jens Peter
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 2, 1870, Frederiksberg C, Denmark.
Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Campus Adamstuen, 0033, Oslo, Norway.
Acta Vet Scand. 2019 Mar 27;61(1):16. doi: 10.1186/s13028-019-0451-7.
Hematologic reference intervals are useful tools for interpretation of laboratory results in swine practice and research. Until now, there were no hematologic reference intervals established for gestating Danish sows and those established in other countries are either out-dated, produced using very few sows or incomplete with few variables. In the past few decades there have been significant increases in litter size due to breeding, as well as the development of new analytical procedures for hematology and statistical procedures for calculating reference intervals. Therefore, hematologic reference intervals representing current pig production and technology are greatly needed. The main objective of this study was to provide updated and complete hematologic reference intervals for Danish sows at mid-gestation. Blood samples were collected at mid-gestation from 248 sows belonging to five commercial herds in Denmark and the samples were analysed for several hematologic variables using the Advia 2120i Hematology System. The reference intervals were calculated according to the guidelines of American Society for Veterinary Clinical Pathology. The reference interval for hemoglobin concentration was 103.1-145.0 g/L. For red blood cells, hematocrit and mean corpuscular volume, the 95% reference intervals were 4.98-7.50 × 10/L, 0.32-0.47 L/L, 57.0-69.3 fL, respectively. The reference intervals established in this study are different from those previously reported in the literature. These reference intervals add to the current knowledge and will be useful in the prevention, diagnosis and treatment of several disease conditions in gestating sow populations.
血液学参考区间是猪医学实践和研究中解读实验室结果的有用工具。到目前为止，丹麦妊娠母猪尚未建立血液学参考区间，而其他国家建立的参考区间要么过时，要么基于很少数量的母猪得出，要么变量不完整。在过去几十年里，由于育种，窝产仔数显著增加，同时血液学新分析程序和计算参考区间的统计程序也得到了发展。因此，非常需要代表当前养猪生产和技术水平的血液学参考区间。本研究的主要目的是为丹麦妊娠中期母猪提供更新且完整的血液学参考区间。在妊娠中期从丹麦五个商业猪群的248头母猪采集血样，并使用Advia 2120i血液学系统对样本进行多种血液学变量分析。参考区间根据美国兽医临床病理学会的指南进行计算。血红蛋白浓度的参考区间为103.1 - 145.0 g/L。红细胞、血细胞比容和平均红细胞体积的95%参考区间分别为4.98 - 7.50×10/L、0.32 - 0.47 L/L、57.0 - 69.3 fL。本研究建立的参考区间与文献中先前报道的不同。这些参考区间丰富了现有知识，将有助于妊娠母猪群体几种疾病状况的预防、诊断和治疗。
