Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran.
Seafood Processing Research Group, School of Agriculture, Shiraz University, Shiraz, Iran.
Sci Rep. 2019 Mar 27;9(1):5287. doi: 10.1038/s41598-019-41589-0.
The present study focused on producing and characterizing a type of biosurfactant (BS) derived from lactic acid bacteria (LAB) and its potential applications in pharmaceutical and food industries due to the preference of employing nonpathogenic organisms in bioprocesses. To this aim, several screening approaches were applied to identify an efficient BS-producing strain from a set of LAB, and Pediococcus dextrinicus SHU1593 was selected as the most operative one. The BS produced by P. dextrinicus was isolated and structurally characterized as a lipoprotein with an approximately equal ratio of lipids (~52% (w/w)) and proteins (47% (w/w)). It reduced the surface tension (ST) of phosphate-buffered saline (PBS) from 72.80 ± 0.10 to 39.01 ± 0.32 mN/m. The results also indicated the potential of developing low-cost strategies aimed at the production of efficient LAB-derived BSs which are structurally and quantitatively similar to the ones obtained from conventional media. Finally, given the physical and functional characterization (i.e. critical micelle concentration (CMC), emulsification index (%E), stability, as well as antimicrobial and anti-adhesive activities) of the BS produced in the present study, it can be introduced as a promising candidate to be employed in plenty of areas in pharmaceutical and food industries.
本研究旨在生产和表征一种源于乳酸菌 (LAB) 的生物表面活性剂 (BS)，并由于在生物工艺中偏好使用非致病性生物，因此将其潜在应用于制药和食品工业。为此，应用了几种筛选方法从一组 LAB 中鉴定出一种有效的 BS 产生菌株，选择了肠膜明串珠菌 SHU1593 作为最有效的菌株。由肠膜明串珠菌产生的 BS 被分离并结构表征为具有大约相等比例的脂质（~52%（w/w））和蛋白质（47%（w/w））的脂蛋白。它将磷酸盐缓冲盐水 (PBS) 的表面张力 (ST) 从 72.80±0.10 降低至 39.01±0.32 mN/m。结果还表明，有可能开发旨在生产结构和数量上与从常规培养基获得的生物表面活性剂相似的高效 LAB 衍生生物表面活性剂的低成本策略。最后，鉴于本研究中产生的 BS 的物理和功能特性（即临界胶束浓度 (CMC)、乳化指数 (%E)、稳定性以及抗菌和抗黏附活性），它可以作为有前途的候选物引入制药和食品工业的许多领域。
