Pashah Zahra, Hekmat Azadeh, Hesami Tackallou Saeed
a Department of Biology , Science and Research Branch, Islamic Azad University , Tehran , Iran.
b Department of Biology , Central Tehran Branch, Islamic Azad University , Tehran , Iran.
Nucleosides Nucleotides Nucleic Acids. 2019;38(4):249-278. doi: 10.1080/15257770.2018.1515440. Epub 2019 Mar 28.
The combination effects of nanodiamonds (NDs) and Paclitaxel (PTX) on the DNA structure were examined. The UV-Visible, steady-state and time-resolved fluorescence spectroscopy, CD, viscosity and zeta potential results showed that PTX + NDs could form a complex via groove binding mechanism. The values of binding constants, ΔG° and ΔH° and ΔS° values showed that PTX + NDs interact strongly with DNA and the hydrophobic force plays main role in this interaction. The and T study indicated the instability of DNA in presence of PTX + NDs. This study demonstrated that NDs could enhance the effect of PTX on DNA structure as well as its affinity and binding to DNA.
研究了纳米金刚石（NDs）和紫杉醇（PTX）对DNA结构的联合作用。紫外可见光谱、稳态和时间分辨荧光光谱、圆二色光谱、粘度和zeta电位结果表明，PTX+NDs可通过沟槽结合机制形成复合物。结合常数、ΔG°、ΔH°和ΔS°值表明，PTX+NDs与DNA强烈相互作用，且疏水作用力在这种相互作用中起主要作用。 和T研究表明，在PTX+NDs存在下DNA不稳定。该研究表明，NDs可增强PTX对DNA结构的影响及其与DNA的亲和力和结合力。
