Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China; Institute for Rational and Safe Medication Practices, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Reproductive Medicine Center, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Fertil Steril. 2019 May;111(5):982-990.e2. doi: 10.1016/j.fertnstert.2019.01.016. Epub 2019 Mar 25.
To evaluate the impact of methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism with clinical data analysis in controlled ovarian hyperstimulation (COH) of infertile women in the Intravenous Infusion Safety Evaluation Center of Hunan Province, People's Republic of China.
Genetic Association Study.
Reproductive medicine clinical.
PATIENT(S): This genetic association study included 722 infertile women who received the standard long treatment protocol with accessible and complete electronic medical records.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): The clinical parameters were obtained from the Intravenous Infusion Safety Evaluation center.
RESULT(S): Basal FSH levels in the TT group were significantly higher than those of the CC group. The FSH levels after down-regulation in the TT group were higher than those of CC/CT genotypes. The TT genotype patients received significantly higher total doses of GnRH agonist and FSH compared with CC/CT genotypes, whereas the total dose of hCG was higher in the CT genotypes compared with the CC/TT genotypes. Further association analysis between hormone levels and COH outcomes indicated significantly negative correlation of basal FSH levels with antral follicle count and number of oocytes as well as the down-regulation FSH levels with the number of metaphase II oocytes and oocytes.
CONCLUSION(S): The MTHFR C677T polymorphism was associated with high doses of ovarian stimulation medications, as well as higher FSH levels. The negative correlation between FSH levels and the number of oocytes suggested that C677T polymorphism may play a role in the poor prognosis of COH oocytes. This needs to be studied in future prospective studies with longer follow-up.
评估亚甲基四氢叶酸还原酶（MTHFR）C677T 多态性与中国湖南省静脉输注安全评价中心不孕妇女控制性卵巢超刺激（COH）的临床数据分析的关系。
遗传关联研究。
生殖医学临床。
本遗传关联研究纳入了 722 名接受标准长治疗方案且可获得完整电子病历的不孕妇女。
无。
临床参数来自静脉输注安全评价中心。
TT 组的基础 FSH 水平明显高于 CC 组。TT 组下调后的 FSH 水平高于 CC/CT 基因型。TT 基因型患者接受的 GnRH 激动剂和 FSH 总剂量明显高于 CC/CT 基因型，而 CT 基因型患者接受的 hCG 总剂量明显高于 CC/TT 基因型。激素水平与 COH 结果之间的进一步关联分析表明，基础 FSH 水平与窦卵泡计数和卵母细胞数呈显著负相关，下调 FSH 水平与中期 II 级卵母细胞和卵母细胞数呈显著负相关。
MTHFR C677T 多态性与卵巢刺激药物的高剂量以及较高的 FSH 水平相关。FSH 水平与卵母细胞数之间的负相关表明，C677T 多态性可能在 COH 卵母细胞的不良预后中发挥作用。这需要在未来具有更长随访时间的前瞻性研究中进一步研究。
