静脉注射免疫球蛋白G对新生大鼠实验性B族链球菌感染期间中性粒细胞动力学的影响。

Effect of intravenous immunoglobulin G on neutrophil kinetics during experimental group B streptococcal infection in neonatal rats.

作者信息

Harper T E, Christensen R D, Rothstein G, Hill H R

出版信息

Rev Infect Dis. 1986 Jul-Aug;8 Suppl 4:S401-8. doi: 10.1093/clinids/8.supplement_4.s401.

DOI:10.1093/clinids/8.supplement_4.s401

Abstract

A modified form of serum immunoglobulin G (pH 4.25) was tested for its effect on neutrophil kinetics and survival rates in neonatal rats with type III, group B streptococcal pneumonia and sepsis. Each of 30 animals received a transthoracic inoculation of 10(5) organisms/g of body weight; all died within 48 hr. When 100, 1,000, or 2,000 mg of immunoglobulin G/kg was administered intraperitoneally at the time of bacterial inoculation, survival rates rose to 20%, 90%, and 100%, respectively. Even when the immunoglobulin preparation was administered intraperitoneally 2 hr after transthoracic inoculation of bacteria, all 19 animals survived. Only seven of 15 animals survived when immunoglobulin administration was delayed for 22 hr. Immunoglobulin facilitated the neutrophil inflammatory response: when immunoglobulin (rather than an albumin control) was administered, neutrophils were released more rapidly from the storage pool and accumulated more quickly at the site of bacterial inoculation. Unlike infected control animals, immunoglobulin recipients did not develop neutropenia or depletion of the neutrophil storage pool.

摘要

对一种改良形式的血清免疫球蛋白G（pH 4.25）进行了测试，观察其对患有III型B组链球菌肺炎和败血症的新生大鼠中性粒细胞动力学及存活率的影响。30只动物每只经胸接种10⁵个细菌/克体重；所有动物在48小时内死亡。在细菌接种时腹腔注射100、1000或2000毫克免疫球蛋白G/千克，存活率分别升至20%、90%和100%。即使在经胸接种细菌2小时后腹腔注射免疫球蛋白制剂，19只动物全部存活。当免疫球蛋白给药延迟22小时时，15只动物中只有7只存活。免疫球蛋白促进了中性粒细胞炎症反应：当给予免疫球蛋白（而非白蛋白对照）时，中性粒细胞从储存池中释放得更快，并且在细菌接种部位积聚得更快。与感染的对照动物不同，接受免疫球蛋白的动物未出现中性粒细胞减少或中性粒细胞储存池耗竭。