University of Campinas - UNICAMP, Graduate Program in Chemical Engineering, Av. Albert Einstein, n 500, Cidade Universitária, 13083-852 Campinas, SP, Brazil.
Federal University of Bahia - UFBA, Graduate Program in Chemical Engineering, Rua Aristides Novis, n 2, Federação, 40210-630 Salvador, BA, Brazil.
Bioresour Technol. 2019 Jul;283:332-339. doi: 10.1016/j.biortech.2019.03.095. Epub 2019 Mar 20.
In the present study, mixed and axenic submerged cultures of Cupriavidus necator and Xanthomonas campestris were performed for simultaneous and individual PHA and XG productions using palm oil (Elaeis guineensis) as substrate. Rotational Central Compound Design (RCCD) was successfully used in the optimization of individual productions of PHA (3.39 g L, Mw = 692.6 kDa) and XG (1.77 g L, Mw = 36.6 × 10 kDa). Novel simultaneous production of PHA (6.43 g L, Mw = 629.2 kDa) and XG (1.98 g L, Mw = 25.0 × 10 kDa), executed in bacterial co-cultivation, revealed to be a successful strategy to increment polymer synthesis, especially PHA. XG bioconversions followed a general trend of lower production in co-culture. Culture configurations also altered polymers properties and characteristics.
在本研究中，使用棕榈油（油棕）作为底物，对鞘氨醇单胞菌和野油菜黄单胞菌进行混合和无菌浸没培养，以同时和分别生产 PHAs 和 XG。旋转中心复合设计（RCCD）成功地用于优化 PHA（3.39g/L，Mw=692.6kDa）和 XG（1.77g/L，Mw=36.6×10kDa）的单独生产。新型同时生产 PHA（6.43g/L，Mw=629.2kDa）和 XG（1.98g/L，Mw=25.0×10kDa），在细菌共培养中进行，被证明是增加聚合物合成的成功策略，特别是 PHA。XG 生物转化的总体趋势是在共培养中产量较低。培养配置也改变了聚合物的性质和特性。
