Mureşan V
Acta Histochem. 1986;79(2):193-8. doi: 10.1016/S0065-1281(86)80081-2.
A sensitive hemagglutination test was developed in order to predict the ability of amine- or hydrazide-derivatized cytochemical markers to interact with cell surface aldehydes in various in vitro and in situ systems. Human red blood cells were modified by glutaraldehyde fixation and subsequent oxidation with sodium periodate, thus exposing free aldehyde residues on their surface, able to form Schiff bases with accessible hydrazide groups on the marker molecule. Fixed and oxidized erythrocytes could be stored for more than 1 year without losing their capacity to be agglutinated by polyhydrazides. The aggregating capacity of hydrazide-enriched molecules correlated well with their binding to oxidized cell surface moieties.
为了预测胺或酰肼衍生的细胞化学标记物在各种体外和原位系统中与细胞表面醛相互作用的能力，开发了一种灵敏的血凝试验。人红细胞通过戊二醛固定并随后用高碘酸钠氧化进行修饰，从而在其表面暴露游离醛残基，能够与标记分子上可及的酰肼基团形成席夫碱。固定和氧化的红细胞可以保存一年以上而不丧失被多酰肼凝集的能力。富含酰肼的分子的聚集能力与其与氧化的细胞表面部分的结合密切相关。
