School of Materials Science & Engineering, College of Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore; Department of Physiology and Immunology Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117456, Singapore.
School of Materials Science & Engineering, College of Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.
Anal Chim Acta. 2019 Jul 11;1061:134-141. doi: 10.1016/j.aca.2019.01.049. Epub 2019 Feb 11.
Photochrome Aptamer Switch Assay (PHASA) is our unique and recently developed fluorescent biosensing platform that exploits the combination of aptamer adaptive binding and photoisomerisation kinetics of stilbenes. The PHASA is based on converting any suitable stilbene-ligand-aptamer complex into a reversible biosensor. Binding affinity, aptamer concentration, and stilbene molecular structure determine the PHASA performance criteria. Consequently, understanding of the structure-activity relationship of the stilbene-ligand conjugate is extremely important when predicting and optimising the biosensor sensitivity. In the present manuscript, new analytical methods relating to calculation of the limit of detection (LOD) of the PHASA are proposed and discussed. Using these methods, it is possible to optimise the independent or dependent physicochemical parameters of the stilbene-ligand conjugates and evaluate their general sensing performance. The proposed analytical methods are based on the inter-dependent relationships between the sensitivity and the range of analyte detection and provide starting conditions for known aptamer binding constants in various PHASA applications.
光致变色适体开关分析（PHASA）是我们独特且最近开发的荧光生物传感平台，利用适体自适应结合和苯乙烯光异构化动力学的结合。PHASA 基于将任何合适的苯乙烯配体适体复合物转化为可逆生物传感器。结合亲和力、适体浓度和苯乙烯分子结构决定了 PHASA 的性能标准。因此，在预测和优化生物传感器灵敏度时，了解苯乙烯配体缀合物的结构-活性关系非常重要。在本手稿中，提出并讨论了与 PHASA 的检测限（LOD）计算相关的新分析方法。使用这些方法，可以优化苯乙烯配体缀合物的独立或相关物理化学参数，并评估其一般传感性能。所提出的分析方法基于灵敏度和分析物检测范围之间的相互依赖关系，并为各种 PHASA 应用中的已知适体结合常数提供了起始条件。
