College of Urban and Environment Sciences, Hunan Provincial Key Laboratory of Comprehensive Utilization of Agricultural and Animal Husbandry Waste Resources, Hunan University of Technology, 88 Taishan Road, Zhuzhou City, Hunan Province 412007, China.
College of Urban and Environment Sciences, Hunan Provincial Key Laboratory of Comprehensive Utilization of Agricultural and Animal Husbandry Waste Resources, Hunan University of Technology, 88 Taishan Road, Zhuzhou City, Hunan Province 412007, China.
Bioresour Technol. 2019 Jul;284:90-97. doi: 10.1016/j.biortech.2019.03.117. Epub 2019 Mar 23.
Anaerobic wastewater potentially was an ideal medium for cultivating microalgae. The coupled effect of ammonium, temperature and pH on lipids accumulation was a core issue during algal culture using anaerobic wastewater. Therefore, their combined effects on Chlorella pyrenoidosa culture and lipids accumulation in anaerobic effluent were investigated. Free ammonia induced from the rising pH and temperature inhibited algal growth, but significantly promoted lipid accumulation. The highest lipids content reached 30.2% when pH rose to 8.3-8.5 (25 °C, ammonium 280 mg/L), which was 1.6-fold higher than that under neutral condition. Moreover, the percentage of unsaturated fatty acids (un-SFAs) increased to 74.8-77.9% at pH 8.3-8.5, whereas it was only 56.1-58.9% under neutral condition. The C18:2 and C18:3 dominated the un-SFAs increase at high pH, typically the percentage of C18:3 increased by 74.5-153.1%. This study provides a potential way for lipid accumulation in algal culture using anaerobic wastewater.
厌氧废水是培养微藻的理想介质。在利用厌氧废水培养藻类的过程中，铵、温度和 pH 值对油脂积累的耦合效应是一个核心问题。因此，本研究考察了它们对蛋白核小球藻培养和厌氧废水中油脂积累的联合作用。升高的 pH 值和温度产生的游离氨抑制藻类生长，但显著促进了油脂积累。当 pH 值升高到 8.3-8.5（25°C，铵 280mg/L）时，油脂含量最高达到 30.2%，比中性条件下高 1.6 倍。此外，在 pH 值为 8.3-8.5 时，不饱和脂肪酸（un-SFAs）的百分比增加到 74.8-77.9%，而在中性条件下仅为 56.1-58.9%。在高 pH 值下，C18:2 和 C18:3 主导了 un-SFAs 的增加，通常 C18:3 的百分比增加了 74.5-153.1%。本研究为利用厌氧废水培养藻类进行油脂积累提供了一种潜在的方法。
