Cozzi M G, Zanini A, Locatelli V, Cella S G, Müller E E
Biochem Biophys Res Commun. 1986 Aug 14;138(3):1223-30. doi: 10.1016/s0006-291x(86)80413-2.
Comparative studies were performed to verify the effect of growth hormone releasing hormone (GHRH) or clonidine (CLON), a compound thought to act via release of endogenous GHRH, in stimulating GH biosynthesis in the pituitary from neonatal and adult rats. In vitro preincubation for 1 h with GHRH (hpGRF-40, 10(-8) M) increased the incorporation of L-[3H]leucine in the electrophoretic band of GH in the pituitary from 10-day-old rats, but not in the gland from adult rats. Ex-vivo treatment with GHRH or CLON for 5 days was effective in stimulating GH biosynthesis only in the pituitary from neonatal rats. These data demonstrate that neonatal somatotropes are particularly sensitive to the GH-synthesizing activity of GHRH or a GHRH-releasing stimulus.
进行了比较研究，以验证生长激素释放激素（GHRH）或可乐定（CLON）（一种被认为通过释放内源性GHRH起作用的化合物）对新生大鼠和成年大鼠垂体中生长激素（GH）生物合成的刺激作用。用GHRH（hpGRF - 40，10^(-8) M）进行1小时的体外预孵育，可增加10日龄大鼠垂体中GH电泳带中L - [3H]亮氨酸的掺入，但对成年大鼠垂体无此作用。用GHRH或CLON进行5天的离体处理仅对新生大鼠垂体中的GH生物合成有刺激作用。这些数据表明，新生期的生长激素细胞对GHRH或GHRH释放刺激的GH合成活性特别敏感。
