Kraljić I
Biochimie. 1986 Jun;68(6):807-11. doi: 10.1016/s0300-9084(86)80096-7.
Indirect methods for the detection of singlet oxygen in dye-sensitized photooxidation based on its interception by some singlet oxygen acceptors in aqueous and micellar solutions are discussed. Mechanistic aspects and some applications of a very sensitive method using p-nitrosodimethylaniline in the presence of imidazole (RNO + imidazole method) are also treated. The technique of competition kinetics with a singlet oxygen quencher N-3 which can serve for the determination of the role of singlet oxygen is discussed as well. Such competition with tryptophan and guanosine shows that these substrates react exclusively or predominantly via the singlet oxygen mechanism in the presence of hematoporphyrin as sensitizing dye.
本文讨论了基于单线态氧在水相和胶束溶液中被某些单线态氧受体拦截，检测染料敏化光氧化中单线态氧的间接方法。还讨论了在咪唑存在下使用对亚硝基二甲基苯胺的非常灵敏的方法（RNO + 咪唑法）的机理方面和一些应用。还讨论了用单线态氧猝灭剂N - 3进行竞争动力学的技术，该技术可用于确定单线态氧的作用。与色氨酸和鸟苷的这种竞争表明，在血卟啉作为敏化染料存在的情况下，这些底物仅通过单线态氧机制反应或主要通过单线态氧机制反应。
