Strbák V, Angyal R, Jurcovicová J, Randusková A
Biol Neonate. 1986;50(2):91-6. doi: 10.1159/000242573.
The role of endogenous thyrotropin-releasing hormone (TRH) in the control of pituitary thyroid-stimulating hormone (TSH) and growth hormone (GH) secretion was studied during postnatal maturation in female Wistar rats. Half of the sucklings in each litter was treated intraperitoneally with either specific rabbit antiserum against TRH or normal rabbit serum (0.1-0.3 ml according to age). All animals were decapitated after 2 h. The presence of anti-TRH activity was checked as a binding of labelled TRH with plasma of the experimental animals. Immunoneutralization of endogenous TRH resulted in a decrease of plasma TSH in 3- to 15-day-old female pups as compared to control littermates. No effect of TRH antibody injection was seen at the ages of 1, 21, 30 and 70 days despite the presence of excess antibody in the plasma. A profound effect of TRH antibody on plasma TSH was seen again at the age of 100 days. Plasma GH in the same animals exhibited a paradoxical increase after TRH immunoneutralization at the age of 5 and 8 days, a decrease was found at the age of 21 days. It was concluded that hypothalamic TRH control of TSH secretion matures early in Wistar rats. Hypothalamic secretion of TRH at the ages of 1, 21, 30, and 70 days is low and(or) its role in TSH regulation is masked by other regulating factors. TRH may play a dual role in the regulation of GH secretion during the postnatal period.
在雌性Wistar大鼠出生后的发育过程中，研究了内源性促甲状腺激素释放激素（TRH）在控制垂体促甲状腺激素（TSH）和生长激素（GH）分泌中的作用。每窝幼崽的一半腹腔注射特异性抗TRH兔抗血清或正常兔血清（根据年龄注射0.1 - 0.3毫升）。2小时后所有动物断头处死。通过标记的TRH与实验动物血浆的结合来检测抗TRH活性的存在。与对照同窝幼崽相比，内源性TRH的免疫中和导致3至15日龄雌性幼崽血浆TSH降低。尽管血浆中存在过量抗体，但在1、21、30和70日龄时未观察到注射TRH抗体的影响。在100日龄时，TRH抗体对血浆TSH再次产生显著影响。相同动物的血浆GH在5日龄和8日龄时TRH免疫中和后呈现出矛盾的增加，在21日龄时发现降低。得出的结论是，下丘脑TRH对TSH分泌的控制在Wistar大鼠中成熟较早。在1、21、30和70日龄时，下丘脑TRH的分泌较低，并且（或者）其在TSH调节中的作用被其他调节因子掩盖。TRH在出生后时期对GH分泌的调节中可能起双重作用。
