Jobin Marie-Lise, Alves Isabel D
Institute for Pharmacology and Toxicology, Rudolf Virchow Center-Bio-Imaging Center, University of Würzburg, Würzburg, Germany.
Chimie et Biologie des Membranes et Nanoobjets, CBMN CNRS UMR 5248, Université Bordeaux 1, Pessac, France.
Methods Mol Biol. 2019;1964:3-15. doi: 10.1007/978-1-4939-9179-2_1.
Membrane-active peptides include a variety of molecules such as antimicrobial (AMP), cell-penetrating (CPP), viral, and amyloid peptides that are implicated in several pathologies. They constitute important targets because they are either at the basis of novel therapies (drug delivery for CPPs or antimicrobial activity for AMPs) or they are the agents causing these pathologies (viral and amyloid peptides). They all share the common property of interacting with the cellular lipid membrane in their mode of action. Therefore, a better understanding of the peptide/lipid (P/L) interaction is essential to help decipher their mechanism of action. Among the different biophysical methods that can be used to fully characterize P/L interactions, differential scanning calorimetry (DSC) allows determining the peptide effect on the lipid phase transitions, a property that reflects the P/L interaction mode. A general protocol for classical DSC experiments for P/L studies will be provided.
膜活性肽包括多种分子，如抗菌肽（AMP）、细胞穿透肽（CPP）、病毒肽和淀粉样肽，它们与多种病理状况有关。它们构成了重要的靶点，因为它们要么是新疗法的基础（CPP用于药物递送，AMP用于抗菌活性），要么是导致这些病理状况的因素（病毒肽和淀粉样肽）。它们在作用方式上都具有与细胞脂质膜相互作用的共同特性。因此，更好地理解肽/脂质（P/L）相互作用对于帮助解读它们的作用机制至关重要。在可用于全面表征P/L相互作用的不同生物物理方法中，差示扫描量热法（DSC）可以确定肽对脂质相变的影响，这一特性反映了P/L相互作用模式。将提供用于P/L研究的经典DSC实验的一般方案。
