使用I2CITC分析复杂相互作用的等温滴定量热法数据。

Analysis of Isothermal Titration Calorimetry Data for Complex Interactions Using I2CITC.

作者信息

Saeed Ibrahim Q, Buurma Niklaas J

机构信息

Department of Chemistry, College of Science, Salahaddin University, Erbil, Kurdistan Region, Iraq.

Physical Organic Chemistry Centre, School of Chemistry, Cardiff University, Cardiff, UK.

出版信息

Methods Mol Biol. 2019;1964:169-183. doi: 10.1007/978-1-4939-9179-2_13.

DOI:10.1007/978-1-4939-9179-2_13

PMID:30929243

Abstract

I2CITC allows the analysis of isothermal titration calorimetry (ITC) data for complex coupled equilibria. Here we describe how, using I2CITC, ITC data for systems involving a self-aggregating ligand and a host offering one or two binding sites can be analyzed, how interaction models can be tested, and how confidence intervals for the optimized parameters can be determined.

摘要

I2CITC可用于分析复杂耦合平衡的等温滴定量热法（ITC）数据。在此，我们描述了如何使用I2CITC来分析涉及自聚集配体和提供一个或两个结合位点的主体的系统的ITC数据，如何测试相互作用模型，以及如何确定优化参数的置信区间。