Ramana Chilakamarti V
Department of Medicine, Dartmouth-Hitchcock Medical Center, Dartmouth Medical School, Lebanon, NH 03766, USA.
Genomics Inform. 2019 Mar;17(1):e8. doi: 10.5808/GI.2019.17.1.e8. Epub 2019 Mar 31.
Alveolar type II cells constitute a small fraction of the total lung cell mass. However, they play an important role in many cellular processes including trans-differentiation into type I cells as well as repair of lung injury in response to toxic chemicals and respiratory pathogens. Transcription factors are the regulatory proteins dynamically modulating DNA structure and gene expression. Transcription factor profiling in microarray datasets revealed that several members of AP1, ATF, NF-kB, and C/EBP families involved in diverse responses were expressed in mouse lung type II cells. A transcriptional factor signature consisting of Cebpa, Srebf1, Stat3, Klf5, and Elf3 was identified in lung type II cells, Sox9+ pluripotent lung stem cells as well as in mouse lung development. Identification of the transcription factor profile in mouse lung type II cells will serve as a useful resource and facilitate the integrated analysis of signal transduction pathways and specific gene targets in a variety of physiological conditions.
肺泡II型细胞在肺细胞总量中占比很小。然而，它们在许多细胞过程中发挥着重要作用，包括转分化为I型细胞以及对有毒化学物质和呼吸道病原体作出反应时修复肺损伤。转录因子是动态调节DNA结构和基因表达的调节蛋白。微阵列数据集中的转录因子谱分析表明，参与多种反应的AP1、ATF、NF-κB和C/EBP家族的几个成员在小鼠肺II型细胞中表达。在肺II型细胞、Sox9+多能肺干细胞以及小鼠肺发育过程中，鉴定出了由Cebpa、Srebf1、Stat3、Klf5和Elf3组成的转录因子特征。鉴定小鼠肺II型细胞中的转录因子谱将成为一种有用的资源，并有助于在各种生理条件下对信号转导通路和特定基因靶点进行综合分析。
