Khattak Ashbala, anNakhli Fouad
Dhahran Eyes Specialist Hospital, Dhahran, Eastern Province, P.O. Box: 39455, Zip: 31942, Saudi Arabia.
Saudi J Ophthalmol. 2019 Jan-Mar;33(1):7-11. doi: 10.1016/j.sjopt.2018.10.005. Epub 2018 Oct 22.
To study the five-year endothelial cell loss in patients having undergone penetrating keratoplasty (PKP) and who received corneal donor tissue from international eye banks.
This retrospective clinical study reviewed outcomes in 41 patients who underwent PKP at a tertiary eye center from February 2008 to July 2011. Standard PKP surgical technique was used for all patients, and graft tissue was supplied in all cases by eye banks in the United States of America. At five years after surgery, measurements were taken of endothelial cell density (ECD), coefficient of variation, hexagonality, donor's age, recipient's age, Death-to-preservation (DP), and preservation time (PT).
The recipients and donors median age was 30.0 years (24.0-35.5 years) and 59.0 years (53.0-61.0 years) respectively; the median DP and PT were 8.0hours.(6.0-10.0hours) and 10.0 days (9.0-11.5 days) respectively. At baseline, the ECD was 2398(2325-2525). At five years after surgery, all of the grafts were found to have survived; the median ECD was 1035 cell/mm (693-1346 cell/mm); the mean coefficient of variation was 35.2 ± 9.8%; and the mean hexagonality was 63.7 ± 24.3%. The overall ECD loss was 56% (95% CI: 50-62%); the ECD loss was 51.3% and 61.2% in corneas from donors younger and older than 60 years respectively (p = 0.056); likewise, the ECD loss was 52.6% and 61.0% in corneas with PT shorter and longer than 10 days respectively (p = 0.289). Although the difference in both cases was not statistically significant, it was clinically important (about 10%).
The ECD after five years in patients undergoing internationally-transported corneal tissue grafts incurred 56% loss; the donor's age and the PT were positively associated with ECD loss.
研究接受穿透性角膜移植术（PKP）并使用国际眼库提供的角膜供体组织的患者五年内的内皮细胞损失情况。
这项回顾性临床研究回顾了2008年2月至2011年7月在一家三级眼科中心接受PKP的41例患者的治疗结果。所有患者均采用标准PKP手术技术，所有病例的移植组织均由美国的眼库提供。术后五年时，测量内皮细胞密度（ECD）、变异系数、六角形细胞比例、供体年龄、受体年龄、死亡至保存时间（DP）和保存时间（PT）。
受体和供体的中位年龄分别为30.0岁（24.0 - 35.5岁）和59.0岁（53.0 - 61.0岁）；中位DP和PT分别为8.0小时（6.0 - 10.0小时）和10.0天（9.0 - 11.5天）。基线时，ECD为2398（2325 - 2525）。术后五年时，所有移植片均存活；中位ECD为1035个细胞/mm²（693 - 1346个细胞/mm²）；平均变异系数为35.2±9.8%；平均六角形细胞比例为63.7±24.3%。ECD总体损失为56%（95%置信区间：50 - 62%）；60岁以下和60岁以上供体角膜的ECD损失分别为51.3%和61.2%（p = 0.056）；同样，保存时间短于和长于10天的角膜ECD损失分别为52.6%和61.0%（p = 0.289）。虽然这两种情况的差异无统计学意义，但在临床上很重要（约10%）。
接受国际运输角膜组织移植的患者术后五年ECD损失56%；供体年龄和保存时间与ECD损失呈正相关。
