Lehmann K R, Kotzin B L, Portanova J P, Santoro T J
Eur J Immunol. 1986 Sep;16(9):1105-10. doi: 10.1002/eji.1830160913.
T cells from murine lupus strains manifest complex defects in interleukin 2 (IL 2) production and receptor expression. The capacity of B cells from such mice to utilize IL 2 as a growth factor has not been previously reported and is examined herein. Anti-Thy-1.2 plus complement-treated spleen cells from 6-8-week-old autoimmune MRL-lpr/lpr mice and from age and sex-matched immunologically normal CBA/J mice were cultured with lipopolysaccharide (LPS) for 36 h and analyzed for the expression of IL 2 receptors using the monoclonal antibody 7D4. The percentage of B cells expressing IL 2 receptors was comparable in MRL-lpr/lpr and CBA/J mice. In contrast to those from CBA/J, BALB/c and (BALB/c X NZW)F1 mice, LPS-stimulated B cells from MRL-lpr/lpr and from (NZB X NZW)F1 mice were capable of proliferating in response to IL 2. Fractionation of MRL-lpr/lpr B cells using Percoll gradient density separation demonstrated that the IL 2-responsive population consisted predominantly of large cells. In addition, unfractionated B cells from MRL-lpr/lpr mice were found to be substantially more responsive to IL 2 than those from CBA/J and BALB/c mice following activation with anti-immunoglobulin plus LPS. The hyper-responsiveness to IL 2 may be a consequence of the state of activation of autoimmune B cells and is of potential importance in the pathogenesis of systemic lupus erythematosus.
来自鼠类狼疮品系的T细胞在白细胞介素2（IL-2）产生和受体表达方面表现出复杂的缺陷。此前尚未报道过此类小鼠的B细胞利用IL-2作为生长因子的能力，本文对此进行了研究。将6至8周龄的自身免疫性MRL-lpr/lpr小鼠以及年龄和性别匹配的免疫正常CBA/J小鼠经抗Thy-1.2加补体处理的脾细胞与脂多糖（LPS）一起培养36小时，并用单克隆抗体7D4分析IL-2受体的表达。表达IL-2受体的B细胞百分比在MRL-lpr/lpr小鼠和CBA/J小鼠中相当。与来自CBA/J、BALB/c和（BALB/c×NZW）F1小鼠的细胞不同，来自MRL-lpr/lpr小鼠和（NZB×NZW）F1小鼠的经LPS刺激的B细胞能够对IL-2作出增殖反应。使用Percoll梯度密度分离法对MRL-lpr/lpr B细胞进行分级分离表明，对IL-2有反应的细胞群主要由大细胞组成。此外，发现用抗免疫球蛋白加LPS激活后，来自MRL-lpr/lpr小鼠的未分级B细胞对IL-2的反应比来自CBA/J和BALB/c小鼠的B细胞强得多。对IL-2的高反应性可能是自身免疫性B细胞激活状态的结果，在系统性红斑狼疮的发病机制中具有潜在重要性。
