Cancer Biomark. 2019;24(4):469-475. doi: 10.3233/CBM-182209.
This study aims to determine the correlation between p-cofilin expression and cisplatin resistance in patients with ovarian cancer, and also to investigate the role of p-cofilin in prognosis.
The ovarian cancer cell line A2780/DDP resistant to cisplatin was prepared. The cell resistance to cisplatin was measured via MTT assay. The cell invasion capacity was identified via Transwell assay. The mRNA expression and protein level was evaluated via semi-quantitative PCR and Western blot, respectively. The tumor tissues of patients with cisplatin-resistant ovarian cancer were collected. The relationship between prognosis and p-cofilin expression was analyzed.
The growth rate of A2780 was similar to that of A2780/DDP. The sensitivity of A2780 to cisplatin was significantly higher than that of A2780/DDP (p< 0.01). The migration capacity of A2780/DDP was significantly increased compared to that of A2780 (p< 0.01), indicating that the cisplatin-resistant cell lines were successfully constructed. Both CFL1 mRNA level and p-cofilin level in A2780/DDP was significantly higher than that in A2780 (p< 0.01). The p-cofilin level in cancer tissues in patients with cisplatin-resistant ovarian cancer was significantly higher than that in patients with cisplatin-sensitive ovarian cancer (p< 0.01). The cisplatin resistance was positively correlated with the p-cofilin expression level (r= 0.802, p= 0.023). The survival time of patients with normal or low level of p-cofilin was significantly longer than that of patients with high expression.
The cisplatin resistance of ovarian cancer is closely related to the expression level of p-cofilin, which affects the prognosis of patients with ovarian cancer.
本研究旨在探讨卵巢癌细胞中 p-cofilin 表达与顺铂耐药的相关性，并探讨 p-cofilin 在预后中的作用。
制备顺铂耐药的卵巢癌细胞系 A2780/DDP。通过 MTT 法测定细胞对顺铂的耐药性。通过 Transwell 测定细胞侵袭能力。通过半定量 PCR 和 Western blot 分别评估 mRNA 表达和蛋白水平。收集顺铂耐药卵巢癌患者的肿瘤组织。分析预后与 p-cofilin 表达的关系。
A2780 的生长速度与 A2780/DDP 相似。A2780 对顺铂的敏感性明显高于 A2780/DDP（p<0.01）。A2780/DDP 的迁移能力明显高于 A2780（p<0.01），表明成功构建了顺铂耐药细胞系。A2780/DDP 中的 CFL1mRNA 水平和 p-cofilin 水平明显高于 A2780（p<0.01）。顺铂耐药卵巢癌患者肿瘤组织中的 p-cofilin 水平明显高于顺铂敏感卵巢癌患者（p<0.01）。顺铂耐药与 p-cofilin 表达水平呈正相关（r=0.802，p=0.023）。p-cofilin 表达水平正常或低的患者的生存时间明显长于高表达的患者。
卵巢癌细胞的顺铂耐药与 p-cofilin 的表达水平密切相关，影响卵巢癌患者的预后。
