Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, Greece; Biomic_Auth, Bioanalysis and Omics Lab, Centre for Interdisciplinary Research of Aristotle University of Thessaloniki, Innovation Area of Thessaloniki, Thermi 57001, Greece.
Biomic_Auth, Bioanalysis and Omics Lab, Centre for Interdisciplinary Research of Aristotle University of Thessaloniki, Innovation Area of Thessaloniki, Thermi 57001, Greece; Laboratory of Forensic Medicine and Toxicology, Department of Medicine, Aristotle University of Thessaloniki, University Campus, 54124 Thessaloniki, Greece.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 May 1;1114-1115:76-85. doi: 10.1016/j.jchromb.2019.03.028. Epub 2019 Mar 23.
Ceratonia siliqua, known as the carob, is considered to be of high nutritional value and of great economic significance due to its unique composition. The beneficial effects of carob against cancer, metabolic syndrome, diabetes, diarrhea, hyperlipidemia and gastro esophageal reflux disease are only a few of its therapeutic actions. Metabolomics-based analysis provides an ultimate tool, for the deciphering of nutritional intervention derived metabolic alterations. In the present study, 16 male Wistar rats were treated with carob powder for a 15-day period. Fecal and urine samples were collected at 5 time points (0, 1, 5, 10 and 15 days). By the applied HILIC-MS/MS method, 63 and 67 hydrophilic metabolites were detected in the fecal and urine samples, respectively, including amino acids, organic acids, sugars, vitamins and other endogenous compounds. A clear group separation based on fecal metabolome was observed after 1 day and 15 days treatment, while only a mild differentiation at day 1 was observed based on urine metabolome. Twenty-one fecal metabolites were responsible for the separation including amino acids and their derivatives, vitamins and organic acids. However, only 7 metabolites were altered in rat urine samples. Metabolic alterations in fecal samples could be attributed to physiological and biochemical adaptations derived from the nutritional intervention. Fecal targeted metabolomics were proven to be suitable for uplifting and highlighting such alterations.
角豆，又称刺槐豆，因其独特的成分而被认为具有很高的营养价值和经济意义。角豆具有抗癌、代谢综合征、糖尿病、腹泻、高血脂和胃食管反流病等多种治疗作用。基于代谢组学的分析为揭示营养干预引起的代谢变化提供了终极工具。在本研究中，16 只雄性 Wistar 大鼠用角豆粉处理 15 天。在 5 个时间点（0、1、5、10 和 15 天）收集粪便和尿液样本。应用 HILIC-MS/MS 方法，在粪便和尿液样本中分别检测到 63 和 67 种亲水代谢物，包括氨基酸、有机酸、糖、维生素和其他内源性化合物。在 1 天和 15 天治疗后，基于粪便代谢组学观察到明显的分组分离，而基于尿液代谢组学仅在第 1 天观察到轻度分化。21 种粪便代谢物负责分离，包括氨基酸及其衍生物、维生素和有机酸。然而，只有 7 种代谢物在大鼠尿液样本中发生了改变。粪便样本中的代谢变化可能归因于营养干预引起的生理和生化适应。粪便靶向代谢组学被证明适用于提升和突出这些变化。
