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三种国际方法与美国公共卫生协会(APHA)方法用于河口水质和贝类中大肠杆菌计数的比较

Comparison of Three International Methods with APHA Method for Enumeration of Escherichia coli in Estuarine Waters and Shellfish.

作者信息

Motes Miles L, McPHEARSON Roland M, DePAOLA Angelo

机构信息

Fishery Research Branch, Food and Drug Administration, Dauphin Island, Alabama 36528.

出版信息

J Food Prot. 1984 Jul;47(7):557-561. doi: 10.4315/0362-028X-47.7.557.

DOI:10.4315/0362-028X-47.7.557
PMID:30934467
Abstract

Three international methods were evaluated for enumerating Escherichia coli in estuarine waters, oysters ( Crassostrea virginica ), mussels ( Mytilus edilus ) and clams ( Mercenaria mercenaria ). Results of the French most probable number (MPN) method, a modification of the MacKenzie, Taylor and Gilbert (1948) method, were obtained within 48 h and compared favorably with those obtained by the standard American Public Health Association (APHA) MPN procedure in all sample types. Results of the Australian Anderson and Baird-Parker plate count method, obtained within 24 h, were significantly lower than those obtained with the standard APHA procedure for all sample types. Results of the British roll tube method, a 24-h direct count method, compared favorably with the standard APHA procedure only for mussels and waters.

摘要

对三种国际方法进行了评估,以测定河口水体、牡蛎(弗吉尼亚巨蛎)、贻贝(紫贻贝)和蛤蜊(硬壳蛤)中的大肠杆菌数量。法国最可能数(MPN)法是对麦肯齐、泰勒和吉尔伯特(1948年)方法的改进,48小时内得出结果,在所有样本类型中,其结果与美国公共卫生协会(APHA)标准MPN程序得出的结果相比更具优势。澳大利亚安德森和贝尔德 - 帕克平板计数法的结果在24小时内得出,在所有样本类型中,该结果显著低于采用APHA标准程序得出的结果。英国滚管法是一种24小时直接计数法,仅在贻贝和水体样本中,其结果与APHA标准程序的结果相比更具优势。

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