Motes Miles L, McPHEARSON Roland M, DePAOLA Angelo
Fishery Research Branch, Food and Drug Administration, Dauphin Island, Alabama 36528.
J Food Prot. 1984 Jul;47(7):557-561. doi: 10.4315/0362-028X-47.7.557.
Three international methods were evaluated for enumerating Escherichia coli in estuarine waters, oysters ( Crassostrea virginica ), mussels ( Mytilus edilus ) and clams ( Mercenaria mercenaria ). Results of the French most probable number (MPN) method, a modification of the MacKenzie, Taylor and Gilbert (1948) method, were obtained within 48 h and compared favorably with those obtained by the standard American Public Health Association (APHA) MPN procedure in all sample types. Results of the Australian Anderson and Baird-Parker plate count method, obtained within 24 h, were significantly lower than those obtained with the standard APHA procedure for all sample types. Results of the British roll tube method, a 24-h direct count method, compared favorably with the standard APHA procedure only for mussels and waters.
对三种国际方法进行了评估,以测定河口水体、牡蛎(弗吉尼亚巨蛎)、贻贝(紫贻贝)和蛤蜊(硬壳蛤)中的大肠杆菌数量。法国最可能数(MPN)法是对麦肯齐、泰勒和吉尔伯特(1948年)方法的改进,48小时内得出结果,在所有样本类型中,其结果与美国公共卫生协会(APHA)标准MPN程序得出的结果相比更具优势。澳大利亚安德森和贝尔德 - 帕克平板计数法的结果在24小时内得出,在所有样本类型中,该结果显著低于采用APHA标准程序得出的结果。英国滚管法是一种24小时直接计数法,仅在贻贝和水体样本中,其结果与APHA标准程序的结果相比更具优势。