载有核黄素的白蛋白亚微米颗粒——制备与表征

Albumin Submicron Particles with Entrapped Riboflavin-Fabrication and Characterization.

作者信息

Suwannasom Nittiya, Smuda Kathrin, Kloypan Chiraphat, Kaewprayoon Waraporn, Baisaeng Nuttakorn, Prapan Ausanai, Chaiwaree Saranya, Georgieva Radostina, Bäumler Hans

机构信息

Institute of Transfusion Medicine, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.

School of Medical Sciences, University of Phayao, Phayao 56000, Thailand.

出版信息

Nanomaterials (Basel). 2019 Mar 25;9(3):482. doi: 10.3390/nano9030482.

Although riboflavin (RF) belongs to the water-soluble vitamins of group B, its solubility is low. Therefore, the application of micro-formulations may help to overcome this limiting factor for the delivery of RF. In this study we immobilized RF in newly developed albumin submicron particles prepared using the Co-precipitation Crosslinking Dissolution technique (CCD-technique) of manganese chloride and sodium carbonate in the presence of human serum albumin (HSA) and RF. The resulting RF containing HSA particles (RF-HSA-MPs) showed a narrow size distribution in the range of 0.9 to 1 μm, uniform peanut-like morphology, and a zeta-potential of -15 mV. In vitro release studies represented biphasic release profiles of RF in a phosphate buffered saline (PBS) pH 7.4 and a cell culture medium (RPMI) 1640 medium over a prolonged period. Hemolysis, platelet activation, and phagocytosis assays revealed a good hemocompatibility of RF-HSA-MPs.

虽然核黄素（RF）属于B族水溶性维生素，但其溶解度较低。因此，微制剂的应用可能有助于克服这一限制RF递送的因素。在本研究中，我们将RF固定在新开发的白蛋白亚微米颗粒中，该颗粒采用氯化锰和碳酸钠在人血清白蛋白（HSA）和RF存在下的共沉淀交联溶解技术（CCD技术）制备。所得含RF的HSA颗粒（RF-HSA-MPs）在0.9至1μm范围内显示出窄的尺寸分布、均匀的花生状形态以及-15 mV的ζ电位。体外释放研究表明，在延长的时间内，RF在pH 7.4的磷酸盐缓冲盐水（PBS）和细胞培养基（RPMI）1640培养基中呈现双相释放曲线。溶血、血小板活化和吞噬作用测定显示RF-HSA-MPs具有良好的血液相容性。