miR-34 rs4938723 T>C多态性在三阴性乳腺癌患者中的预后意义

Prognostic significance of miR-34 rs4938723 T > C polymorphism in triple negative breast cancer patients.

作者信息

Tsiakou Andriani, Zagouri Flora, Zografos Eleni, Samelis George, Gazouli Maria, Kalapanida Despoina, Giannos Aris, Marinopoulos Spyros, Dimitrakakis Konstantinos, Lazaris C Andreas, Rigopoulos Dimitrios, Zografos George

机构信息

Department of Dermatology, 'Andreas Syggros' Hospital of Cutaneous & Venereal Diseases, University of Athens, Athens, Greece.

Department of Clinical Therapeutics, Alexandra Hospital, University of Athens, Athens, Greece.

出版信息

Clin Biochem. 2019 Jun;68:9-14. doi: 10.1016/j.clinbiochem.2019.03.009. Epub 2019 Mar 29.

DOI:10.1016/j.clinbiochem.2019.03.009

PMID:30935968

Abstract

OBJECTIVES

The aim of this was the assessment of the prognostic role of the rs4938723 C > T polymorphism of the miR-34 in triple negative breast cancer patients.

METHODS

Therefore formalin fixed paraffin embedded tissue samples from 114 triple negative breast cancer patients and blood samples from 124 healthy donors were genotyped and subsequently extensive statistical analysis was performed in order to investigate the clinical value of this polymorphism in triple negative breast cancer.

RESULTS

Our statistical analysis disclosed that the majority of patients harboring ductal breast carcinoma (69.4%) have the TC or CC genotypes (P = .020). Moreover the survival of the patients was significantly correlated with the occurrence of the TC or CC alleles (P < .001). Regarding the correlation of miR-34 polymorphisms with patients' survival we found that women with TC or CC single nucleotide polymorphisms were characterized by shorter disease free survival intervals (P = .05). Furthermore triple negative breast cancer patients with TC/CC genotype exhibited shorter overall survival intervals as disclosed by Kaplan Meier analysis (P < .001) and Cox regression analysis (HR = 3.2, %95 CI = 2.0-5.5, P = .008). Stratified Kaplan-Meier analysis showed that the women harboring the TC or CC genotype along with the ductal histology had significantly shorter survival (P < .001). This result was also confirmed by Univariate Cox regression analysis, which showed that women ductal breast cancer and TC or CC genotype are of worse prognosis (HR = 2.35, %95 CI = 2.1-4.65, P = .003).

CONCLUSIONS

In conclusion, we found that the TC and CC alleles are associated with unfavorable prognosis in triple negative breast cancer patients.

摘要

目的

本研究旨在评估miR-34的rs4938723 C>T多态性在三阴性乳腺癌患者中的预后作用。

方法

因此，对114例三阴性乳腺癌患者的福尔马林固定石蜡包埋组织样本和124例健康供者的血液样本进行基因分型，随后进行广泛的统计分析，以研究该多态性在三阴性乳腺癌中的临床价值。

结果

我们的统计分析显示，大多数患有导管乳腺癌的患者（69.4%）具有TC或CC基因型（P = 0.020）。此外，患者的生存率与TC或CC等位基因的出现显著相关（P < 0.001）。关于miR-34多态性与患者生存率的相关性，我们发现具有TC或CC单核苷酸多态性的女性无病生存期较短（P = 0.05）。此外，Kaplan Meier分析显示，TC/CC基因型的三阴性乳腺癌患者总生存期较短（P < 0.001），Cox回归分析显示（HR = 3.2，95%CI = 2.0 - 5.5，P = 0.008）。分层Kaplan-Meier分析显示，具有TC或CC基因型以及导管组织学的女性生存期明显较短（P < 0.001）。单因素Cox回归分析也证实了这一结果，该分析显示导管乳腺癌女性和TC或CC基因型预后较差（HR = 2.35，95%CI = 2.1 - 4.65，P = 0.003）。