蝌蚪血清中半胱天冬酶 - 3的活性（a）作为细胞凋亡作用的标志物以及肿瘤诱导的白化大鼠上皮细胞中总细胞毒性T淋巴细胞的标志物。

Tadpole serum activity ( a) in caspase-3 as a marker of the role of apoptosis and total cytotoxic T lymphocytes in albino rats' epithelial cells induced by neoplasia.

作者信息

Purnama M T E, Rahmaningtyas I H, Pratama A R, Prastika Z, Kartikasari A M, Cahyo N P D

机构信息

Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo Campus C Surabaya, East Java 60115, Indonesia.

Department of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo Campus C Surabaya, East Java 60115, Indonesia.

出版信息

Vet World. 2019 Jan;12(1):63-67. doi: 10.14202/vetworld.2019.63-67. Epub 2019 Jan 10.

DOI:10.14202/vetworld.2019.63-67

PMID:30936655

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6431812/

Abstract

AIM

This study was conducted to examine the tadpole's serum activity () in caspase-3 as a marker of the role of apoptosis and total cytotoxic T lymphocyte (CTL) in albino rats' epithelial cells induced by neoplasia. Tadpole serumcontains thyroxine hormone that may cause the metamorphosis process and control cell proliferation.

MATERIALS AND METHODS

Male rats were induced by 7,12-dimethylbenz (α)anthracene (DMBA) 20 mg/rats twice every week over 5 weeks to stimulate skin neoplasia. Tadpole serum injected intracutaneously after neoplasia is known. The negative control group (C-) was not exposed to DMBA and tadpole serum, while the positive control group (C+) was exposed to DMBA. Treatment groups (T1, T2, and T3) were exposed DMBA and tadpole serum 100%, 75%, and 25%/rat/day, respectively. Samples of skin organ were be made preparations immunohistochemistry interacted with caspase-3 and CTL antibody as the marker.

RESULTS

Based on the result, immunohistochemistry from skin neoplasia and given therapy of tadpole serum show that Treatment 1 was the highest caspase-3 and CTL expression. The result of caspase-3 expression in C-, C+, T1, T2, and T3 was 0.00±0.000, 0.70±0.141, 2.00±0.283, 1.10±0.424, and 1.15±0.495, respectively. The result of CTL expression in C-, C+, T1, T2, and T3 was 0.10±0.200, 1.00±0.230, 2.10±0.529, 1.70±0.258, and 1.35±0.443, respectively.

CONCLUSION

It can be concluded from the study that tadpole serum () 100% concentration can increase caspase-3 and total CTL in albino rats' epithelial cells induced by neoplasia.

摘要

目的

本研究旨在检测蝌蚪血清中半胱天冬酶 -3 的活性（），作为肿瘤诱导的白化大鼠上皮细胞凋亡作用及总细胞毒性 T 淋巴细胞（CTL）的标志物。蝌蚪血清含有甲状腺激素，可能引发变态过程并控制细胞增殖。

材料与方法

雄性大鼠每周两次，每次 20mg/只，连续 5 周注射 7,12 - 二甲基苯并（α）蒽（DMBA）以诱导皮肤肿瘤。在确认发生肿瘤后，经皮内注射蝌蚪血清。阴性对照组（C-）未接触 DMBA 和蝌蚪血清，而阳性对照组（C+）接触 DMBA。治疗组（T1、T2 和 T3）分别以 100%、75%和 25%/只·天的剂量接触 DMBA 和蝌蚪血清。制备皮肤器官样本，进行免疫组织化学检测，使其与作为标志物的半胱天冬酶 -3 和 CTL 抗体相互作用。

结果

基于结果，皮肤肿瘤的免疫组织化学及蝌蚪血清治疗显示，治疗 1 组半胱天冬酶 -3 和 CTL 表达最高。C-、C+、T1、T2 和 T3 组中半胱天冬酶 -3 的表达结果分别为 0.00±0.000、0.70±0.141、2.00±0.283、1.10±0.424 和 1.15±0.495。C-、C+、T1、T2 和 T3 组中 CTL 的表达结果分别为 0.10±0.200、1.00±0.230、2.10±0.529、1.70±0.258 和 1.35±0.443。