The occurrence of disinfectant and antibiotic-resistant genes in isolated from chickens in Egypt.

AIM

This work aimed to determine the occurrence of antibiotic and disinfectant resistance genes in isolated from chickens in Egypt.

MATERIALS AND METHODS

Organs (liver, lung, heart, yolk sac, and bone marrow) of 1500 chicken samples were collected from diseased chickens suffered from colibacillosis with PM findings as CRD, diarrhea and omphalitis from different governorates of Egypt as: Giza, EL-Bahira, Fayoum, El-Dakahlia, El-Ismalia, and El-Sharkia during 2015-2016. These samples were labeled and transported immediately on ice to the Reference laboratory for quality control on poultry production (RLQP). The samples were cultured onto MacConkey agar and Eosin Methylene Blue Agar. Isolation and identification of the were performed based on morphology, cultural, staining, and biochemical properties. Antimicrobial resistance test was carried out using disk diffusion method. The PCR employing and were carried out for detection of these genes in isolated .

RESULTS

The prevalence of in chicken was 34%. Predominant serotypes of which serologically identified were O128, O111, O44, O158, and O2. Antibiotic susceptibility test of revealed that 100% of isolates were resistant to ampicillin, erythromycin, and sulfamethoxazole-trimethoprim, while 73.53% and 38.23% of them were sensitive for colistin sulfate and levofloxacin, respectively. Antibiotic resistance genes as gene were tested for isolated and detected by incidence rate of 91.18%. resistance genes resembling as and genes were detected in isolated . 70.6% and 14.7%, respectively.

CONCLUSION

. isolated from chickens in Egypt was carried and antibiotic-resistant genes that affect the poultry industry.