Meng Li, Gao Hui, Chen Bin, Liu Peng-Peng, Shan Guo-Shun, Zhang Fan, Jia Tian-Zhu
School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.
J Anal Methods Chem. 2019 Feb 26;2019:6454252. doi: 10.1155/2019/6454252. eCollection 2019.
A rapid and sensitive quantitative analytical method was established for the simultaneous determination of five chromones (prim-O-glucosylcimifugin, cimifugin, 4'-O--D-glucosyl-5-O-methylvisamminol, 5-O-methylvisammiol, and sec-o-glucosylhamaudol) in the plasma of RS-treated rats for the first time using ultra performance liquid chromatography- (UPLC-) tandem mass spectrometry. The Waters Acquity UPLC BEH C18 (50 mm × 2.1 mm, 1.7 m) was used as the chromatographic column, 0.1% formic acid water and 0.1% formic acid acetonitrile comprised the mobile phases, and all samples were determined under positive ion mode. The results showed that all analytes had good linearity ( > 0.9902), between-day and within-day precisions less than 15%, accuracy between -5.50% and 5.53%, and extraction recovery between 88.26% and 97.65%. Both the matrix effect and stability met the requirements. This method was successfully applied for the comparative pharmacokinetics of five active components of RS in normal and febrile rats. The results showed that the pharmacokinetic behavior of RS extract significantly differed between the two types of rats.
首次建立了一种快速灵敏的定量分析方法，采用超高效液相色谱-串联质谱法同时测定RS处理大鼠血浆中的5种色原酮（prim-O-葡萄糖基升麻苷、升麻苷、4'-O-β-D-葡萄糖基-5-O-甲基维斯阿米醇、5-O-甲基维斯阿米醇和sec-O-葡萄糖基哈马豆素）。采用Waters Acquity UPLC BEH C18色谱柱（50 mm×2.1 mm，1.7 μm），流动相为0.1%甲酸水和0.1%甲酸乙腈，所有样品在正离子模式下测定。结果表明，所有分析物线性关系良好（r>0.9902），日间和日内精密度均小于15%，准确度在-5.50%至5.53%之间，提取回收率在88.26%至97.65%之间。基质效应和稳定性均符合要求。该方法成功应用于正常大鼠和发热大鼠中RS 5种活性成分的比较药代动力学研究。结果表明，RS提取物在两种类型大鼠中的药代动力学行为存在显著差异。
