Reichenspurner H, Ertel W, Reichart B, Kemkes B M, Jamieson S W, Hammer C, Brendel W
J Surg Res. 1986 Sep;41(3):301-7. doi: 10.1016/0022-4804(86)90040-5.
A method of hetero-orthotopic heart-lung transplantation is described in the dog. The model was developed to study patterns of rejection in the transplanted heart and lung, since the dog will not survive bilateral pneumonectomy and loss of the Hering-Breuer reflex. After removal of the recipient's left lung the donor heart and left lung are explanted en bloc. End-to-end connection is made of both left main bronchi, and the donor aorta is joined end-to-side with the recipient's descending aorta. An atrio-atrial anastomosis is performed between the recipient's left and the donor's right atrium. Four experiments were done to develop the surgical technique and 10 long-term studies were performed to investigate rejection patterns. The average survival rate of these animals was 28.5 +/- 8.3 days, ranging from 5 to 53 days. Causes of death were not due to operative complications. Heterotopic heart-lung transplantation is an uncomplicated surgical method which does not require cardiopulmonary bypass or anticoagulation and allows the investigator to study heart and lung grafts in dogs for long-term periods after surgery. Bronchoscopy, bronchoalveolar lavage, and heart and lung biopsies of both donor and recipient organs can be performed.
本文描述了一种在犬类中进行异位心肺移植的方法。开发该模型是为了研究移植心肺的排斥模式，因为犬类在双侧肺切除和赫林 - 布雷尔反射丧失后无法存活。切除受体的左肺后，将供体心脏和左肺整体取出。对两根左主支气管进行端端连接，供体主动脉与受体降主动脉进行端侧吻合。在受体左心房和供体右心房之间进行心房 - 心房吻合。进行了四项实验以完善手术技术，并进行了十项长期研究以调查排斥模式。这些动物的平均存活率为28.5 +/- 8.3天，范围从5天到53天。死亡原因并非手术并发症所致。异位心肺移植是一种简单的手术方法，不需要体外循环或抗凝，并且使研究人员能够在手术后对犬类的心肺移植物进行长期研究。可以对供体和受体器官进行支气管镜检查、支气管肺泡灌洗以及心肺活检。
