Khashim Zenith, Fitzgerald Seán, Kadirvel Ramanathan, Dai Daying, Doyle Karen M, Brinjikji Waleed, Kallmes David F
Department of Radiology, Mayo Clinic, Rochester, USA.
Department of Physiology and CÚRAM-Centre for Research in Medical Devices, National University of Ireland Galway, Galway, Ireland.
Interv Neuroradiol. 2019 Oct;25(5):502-507. doi: 10.1177/1591019918819707. Epub 2019 Apr 2.
Bacteria and bacterial components have been associated with the activation of coagulation factors and initiating the blood clot formation. The aim of this study was to investigate whether bacterial populations are present in clots retrieved from patients that have suffered a large vessel occlusion acute ischemic stroke (AIS).
Clot samples were collected from 20 AIS patients who underwent clot retrieval with mechanical thrombectomy. Patient clinical demographic details were noted. Expression of bacterial 16S rDNA was analyzed by standard and real-time polymerase chain reaction (PCR). Gram staining was performed to identify Gram-positive and Gram-negative bacteria.
Both the real-time and standard PCR demonstrated no expression of 16S rDNA in any of the 20 clots samples from AIS patients. Gram staining results showed no expression of Gram-positive or Gram-negative bacteria present in the clot samples.
Our current study found no bacteria populations in the clots of AIS patients.
细菌及细菌成分与凝血因子的激活及血栓形成的启动有关。本研究的目的是调查在因大血管闭塞急性缺血性卒中（AIS）而接受取栓治疗的患者所取出的血栓中是否存在细菌群落。
从20例接受机械取栓治疗的AIS患者中收集血栓样本。记录患者的临床人口统计学细节。通过标准和实时聚合酶链反应（PCR）分析细菌16S rDNA的表达。进行革兰氏染色以鉴定革兰氏阳性菌和革兰氏阴性菌。
实时和标准PCR均显示，来自AIS患者的20个血栓样本中均未检测到16S rDNA的表达。革兰氏染色结果显示，血栓样本中未检测到革兰氏阳性菌或革兰氏阴性菌的表达。
我们目前的研究发现AIS患者的血栓中不存在细菌群落。
